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    influenza-a-virus-m2-protein-antibody-14c2-ab5416.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Influenza
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Anti-Influenza A Virus M2 Protein 抗体 [14C2] (ab5416)

  • Datasheet
  • SDS
Reviews (2)Q&A (17)References (62)

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Abpromise

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Immunocytochemistry - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

    Key features and details

    • Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
    • Suitable for: ICC
    • Reacts with: Dog, Influenza A
    • Isotype: IgG1

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
    二次抗体
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    一次抗体
    Product image
    FITC Anti-Influenza A Virus Nucleoprotein antibody [D67J] (ab210526)

    関連製品

    製品の概要

    • 製品名

      Anti-Influenza A Virus M2 Protein antibody [14C2]
      Influenza A Virus M2 Protein 一次抗体 製品一覧
    • 製品の詳細

      Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
    • 由来種

      Mouse
    • アプリケーション

      適用あり: ICCmore details
    • 種交差性

      交差種: Dog, Influenza A
    • 免疫原

      Full length protein corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

    • エピトープ

      Detects the N-terminal of the Influenza A Virus M2 Protein.
    • ポジティブ・コントロール

      • ICC/IF: Infected MDCK cells.
    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 99% PBS, 0.1% BSA
    • Concentration information loading...
    • 精製度

      Protein G purified
    • 一次抗体 備考

      Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
    • ポリ/モノ

      モノクローナル
    • クローン名

      14C2
    • アイソタイプ

      IgG1
    • 軽鎖の種類

      kappa
    • 研究分野

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Influenza

    関連製品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab5416の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC (1)
    Use a concentration of 1 µg/ml.
    特記事項
    ICC
    Use a concentration of 1 µg/ml.

    ターゲット情報

    • 関連性

      The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
    • 細胞内局在

      Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
    • 参照データベース

      • Entrez Gene: 1461002 Influenza A
      • Entrez Gene: 956528 Influenza A
      • SwissProt: A0A2R3YRM7 Influenza A
      • SwissProt: P06821 Influenza A
      • SwissProt: Q67179 Influenza A
      • 別名

        • Influenza A virus matrix protein M2 antibody
        • M antibody
        • M2 antibody
        • Matrix protein 2 antibody
        • Membrane ion channel M2 antibody
        • Membrane protein M2 antibody
        • Proton channel protein M2 antibody
        see all

      画像

      • Immunocytochemistry - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)
        Immunocytochemistry - Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

        Immunofluorescence staining of infected dog MDCK cells using ab5416.

      プロトコール

      • Flow cytometry protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • SDS download

      • Datasheet download

        Download

      参考文献 (62)

      ab5416 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5416 は 62 報の論文で使用されています。

      • Pila-Castellanos I  et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274
      • Colant N  et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
      • Zhang W  et al. Trojan Horse Delivery of 4,4'-Dimethoxychalcone for Parkinsonian Neuroprotection. Adv Sci (Weinh) 8:2004555 (2021). PubMed: 33977069
      • Durgan J  et al. Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine. Mol Cell 81:2031-2040.e8 (2021). PubMed: 33909989
      • Blokhina EA  et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-10 of 19 Abreviews or Q&A

      Question

      Please send me a quote for 3 vials of BSA and azide free influenza A antibody.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Oct 23 2012

      Answer

      Thank you very much for your patience.

      Finally, I have a price quote and custom made confirmation from laboratory. We are happy to provide this product at lower cost than ab5416. The price would be £194 per vial or £582 for 3 vials of this product, excluding shipping charges which will be £16 for UK and 21 Euros for Germany.

      Could you let me know if you want to go ahead with the order? I will then be happy to publish the datasheet of this custom made product.

      Please note for proper quotation I would need the billing and shipping address.

      I will look forward to hearing from you soon.

      Read More

      Abcam Scientific Support

      Answered on Oct 23 2012

      Question

      Please send me a quote for 3 vials of BSA and azide free influenza A antibody.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Oct 22 2012

      Answer

      Thank you for your email.

      I have sent a request to laboratory for quote; I will send you the details soon.

      Many thanks for having patience!

      Read More

      Abcam Scientific Support

      Answered on Oct 22 2012

      Question

      I would like to buy two vials of the BSA and azide free monoclonal antibody against the Influenza A M2 protein (see below).

      vial volume: 100µL

      antibody concentration: 1mg/mL stored in PBS

      Is it possible to get an offer?

      Thank you!

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Oct 18 2012

      Answer

      Thank you for your email.

      I just spoken to my colleague in laboratory; they have suggested that in order to justify the production cost for making it BSA and azide free at least 3 vials should be ordered. Could you let me know if you will buy 3 vials and then I will send you a quote.

      I will look forward to hearing from you soon. Let me know if you have any questions and concerns.

      Read More

      Abcam Scientific Support

      Answered on Oct 18 2012

      Question

      I bought the antibody (ab5416) month ago. But the antibody is stored in a 1mg/mL BSA solution. Do you have this antibody also in a BSA free solution?

      In an earlier search I found the ab96689.

      Best regards!

      Read More

      Abcam community

      Verified customer

      Asked on Oct 10 2012

      Answer

      Thank you for contacting us.

      We can provide this product, BSA and azide free as a special request. Could you let me know how many vials you would like to buy?

      Looking forward to hearing from you soon.

      Read More

      Abcam Scientific Support

      Answered on Oct 10 2012

      Question

      Customer kindly called to inquire whether this product would recognize the Influenza A PR8 strain.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 21 2012

      Answer

      Thank you for contacting us.

      Our Influenza A M2 Monoclonal Antibody (14C2) - product #ab5416 was shown to detect influenza A virus M2 strain A/PR/8/34 in the following reference:



      Zebedee SL, Lamb RA"Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions."J Virol. 1988 Aug;62(8):2762-72.
      (http://www.ncbi.nlm.nih.gov/pubmed/2455818)

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Abcam Scientific Support

      Answered on Jun 21 2012

      Question

      Thank you very much for your help!

      1. I measured the OD280 of the various steps from my sample using the Nandrop ND1000. I did not dilute the samples (except for the original antibody stock) prior to measuring.
      Raw A280 values:
      Original antibody solution (50% concentration) = 0.712
      Unbound fraction = 1.46
      Wash1 = -0.022
      Wash2 = -0.014
      Wash3 = 0.022
      Wash4 =0.015
      Elution1 =0.069
      Elution2 = -0.013
      Elution3 = 0.038
      Elution4 = -0.032

      2. Please confirm the volumes used. When calculating recoveries and absolute amounts of material we need to know the volume as well as the concentration.
      For the volumes used, I used 100uL of the antibody and added 10uL of binding buffer to it. This was mixed and applied to the resin in the spin column. The resin itself was reconstituted using 0.5mL of wash buffer. The mixture was incubated at 25C in a thermomixer for 1 hour.
      For the wash steps, I used 0.5mL wash buffer 4 times, and spun down 30s as stated in the protocol
      For the elution steps, I added 100uL of elution buffer to the spin catridge containing the resin and protein, and incubated this at 25C for 2 min, in a thermomixer. This was then spun down for 30 sec. I then added 25uL of the neutralization buffer to the eluate, and stored this on ice for the rest of the procedure. I repeated this elution step 4 times.

      Thank you for your help.
      Regards,

      Read More

      Abcam community

      Verified customer

      Asked on Mar 20 2012

      Answer

      Thank you foryour reply and forkindly providing the requested information.

      I have investigated this with our source for the antibody and reviewed the details. It is worth consideringthat the Nandrop will be unable to distinguish between IgG and BSA. Also the OD280 nm values are very low and a little variable.It is difficult to draw an absolute conclusion but this does indicate that the antibody did not bind to the column. However, this can be confirmed with further investigation.

      Unfortunately, experience has shown that the measurement of antibody recovery using the nanodrop is not always very accurate.We would therefore recommendto confirmrecovery of the antibody by an alternative method. For example, a 1D non reducing gel would provide an indication of the amount of antibody and its quality. There shouldbe asingle antibody band of approximately 160 kDa. The intensity of this band will be related to the amount of antibody recovered.

      If the recovery is in fact found to be low then it means that for some reason the antibody has failed to bind to the resin. The antibody will therefore be in the flow through. Again a 1D non reducing gel of the flow through sample could confirm this (the material will have two bands IgG and BSA). In this case you could repeat the binding to the resin after adding a second volume of binding buffer. The second addition of binding buffer will make sure that the pH of the antibody is definitely correct (pH 8.0) and that the ionic strength of the antibody buffer is sufficiently raised to promote binding. The kits are provided with sufficient excess buffers to enablethis procedure. The addition of more binding buffer has proved very successful in the past when dealing with troublesome mouse antibodies.

      I hope this will be helpful to you. Please dolet me know how you get on. If you still have some concerns, please do not hesitate to contact me with further information and details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Mar 20 2012

      Question

      Hi, I would like to know whether this antibody is also a neutralizing antibody,i.e. preventing cells from Flu virus infection once ab5416 is added to the cell culture. Thx

      Read More

      Abcam community

      Verified customer

      Asked on Feb 28 2012

      Answer

      Thank you for contacting Abcam regarding this,
      I have had the chance to speak with the lab about your question. We have not tested whether this antibody can be used as a neutralizing antibody. The authors in the following paper used this antibody in a blocking assay. They found out that "The level of M2 surface expression in A/Udorn virus-infected MDCK cells was found to be reduced to ∼60% of control levels in cells incubated with the 14C2 antibody. In contrast, M2 surface expression levels in A/WSN virus-infected cells were decreased by only ∼5-15%, and A/WSN virus assembly appeared to be unaffected by the M2 antibody treatment." It appears that the blocking is also virus strain dependent.

      http://www.sciencedirect.com/science/article/pii/S0042682285714985

      I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More

      Abcam Scientific Support

      Answered on Feb 28 2012

      Question

      I am using a 1% concentration of Sodium Deoxycholate in a buffer composed of 10 mM Tris, 150 m M NaCl,PH 8. SDO forms a viscous gel and precipitates!!!

      To my knowledge, SDO precipitates in a PH lower than 7 but PH of my buffer is 8.

      I would be much grateful if you help me with this problem. I have heard about NaCl concentrations above 0.1% which makes SDO insoluble. Is that correct?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 31 2012

      Answer

      I would suggest to prepare buffers that are recommended in the following Western Blotting protocol : https://www.abcam.com/index.html?pageconfig=resource&rid=13045 .

      Read More

      Abcam Scientific Support

      Answered on Jan 31 2012

      Question

      I have a question regarding M2 Antibody I have ordered to you... Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416) Actually I want to use this antibody against my virus which is Influenza A Virus (A/New Jersey/8/1976(H1N1) But this antibody is against Influenza A virus (A/WSN/1933(H1N1) Could you please let me know if it is matched with my target protein from conformation point of view? because I have used it for FITC and HRP conjugated antibodies, but no result detected... I think this primary is not match with my target!!! and also ask this question from Abcam Company: If they can suggest or provide me any suitable antibody for this detection? Thank you very much .

      Read More

      Abcam community

      Verified customer

      Asked on Dec 23 2011

      Answer

      Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see the results below. Indeed, the alignement is very good. However as this is a monoclonal antibody, we do not know the epitope and can therefore not guarantee crossreactivity. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111223-121219-0395-63524695-pg We do have two other antibodies against the Influenza A Virus M2 protein, the ab56086 and the ab94925. I have already done the alignement of the immunogen of ab56086 with your protein of interest. The overlapp is 100%. I would therefore expect that this antibody does recognize your protein as well. xxx I am checking with the laboratory for the alingment of ab94925 with your protein (http://www.uniprot.org/uniprot/A5GX42) and will get back to you. If the customer would like to test the ab56086 on the Jersey Strain, I would be pleased to provide also a testing discount (www.abcam.com/collaborationdiscount). Please let me know if you would like to know more about this testing discount. Please do not hesitate to contact me, should you or the customer have any other question or concern.

      Read More

      Abcam Scientific Support

      Answered on Dec 23 2011

      Question

      Can you provide the exact epitope sequence in the N terminal region where this product, Influenza A Virus M2 Protein antibody [14C2] (MA1-082), detects? Also, does this product detect all sub-types (e.g. H1N1, H5N1)?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 07 2011

      Answer

      Thank you for your enquiry. This product was created against whole M2 protein purified from A/WSN/33-infected CV1 cell lysate. Unfortunately, the exact epitope for this polyclonal antibody is not known at this time. The antibody was not tested against all subtypes, but the M2 protein should be identical in the influenza A virus subtypes.

      Read More

      Abcam Scientific Support

      Answered on Jan 07 2011

      1-10 of 19 Abreviews or Q&A

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