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    indoleamine-2-3-dioxygenase-antibody-ab106134.pdf

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Signal Transduction Metabolism Amino Acids
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Anti-Indoleamine 2, 3-dioxygenase 抗体 (ab106134)

  • Datasheet
Submit a review Q&A (5)References (17)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (ab106134)

    Key features and details

    • Rabbit polyclonal to Indoleamine 2, 3-dioxygenase
    • Suitable for: WB, IHC-Fr, IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    こちらの製品もご検討ください

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    Human Anti-Toxoplasma gondii IgG ELISA Kit (ab108776)
    タンパク質
    Product image
    Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)
    一次抗体
    Product image
    Anti-CD161 antibody [EPR21236] (ab234107)

    関連製品

    製品の概要

    • 製品名

      Anti-Indoleamine 2, 3-dioxygenase antibody
      Indoleamine 2, 3-dioxygenase 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Indoleamine 2, 3-dioxygenase
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WB, IHC-Fr, IHC-Pmore details
    • 種交差性

      交差種: Mouse, Rat, Human
    • 免疫原

      A mixture of 3 peptides derived from Rat Indoleamine 2, 3-dioxygenase: MPHSQISPAEGSRRILEEY (1-19), LFSFPGGDCDKGFFLVSLMVE (155-176), and VKPSKQKPMGGHKSEEPS (359-376), conjugated to KLH.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • バッファー

      Preservative: 0.02% Sodium azide
      Constituents: 0.1% BSA, PBS
    • Concentration information loading...
    • 精製度

      Protein G purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Metabolism
      • Amino Acids
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Amino acid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human Indoleamine 2, 3-dioxygenase protein (Active) (ab219439)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab106134の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    WB
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    IHC-P
    1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
    • パスウェイ

      Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
    • 配列類似性

      Belongs to the indoleamine 2,3-dioxygenase family.
    • Target information above from: UniProt accession P14902 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 3620 Human
      • Entrez Gene: 15930 Mouse
      • Entrez Gene: 66029 Rat
      • Omim: 147435 Human
      • SwissProt: P14902 Human
      • SwissProt: P28776 Mouse
      • SwissProt: Q9ERD9 Rat
      • Unigene: 840 Human
      • Unigene: 392 Mouse
      • Unigene: 64513 Rat
      see all
    • 別名

      • 3-dioxygenase antibody
      • I23O1_HUMAN antibody
      • IDO 1 antibody
      • IDO antibody
      • IDO-1 antibody
      • IDO1 antibody
      • INDO antibody
      • indolamine 2,3 dioxygenase antibody
      • Indole 2 3 dioxygenase antibody
      • indoleamine 2 3 dioxygenase 1 antibody
      • indoleamine 2 3 dioxygenase antibody
      • Indoleamine 2,3-dioxygenase 1 antibody
      • Indoleamine pyrrole 2 3 dioxygenase antibody
      • Indoleamine-pyrrole 2 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (ab106134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody (ab106134)

      Rat astrocytes labeled with ab106134 at 1/50 dilution. The polyclonal antibody recognizes rat indoleamine 2,3-dioxygenase. Primary antibody binding was determined using anti-rabbit immunoglobulin VectastainElite ABC kit. Counterstained with hematoxylin. 

      Heat (at 60°C in 10 mM sodium citrate, pH6.0, for 60 minutes) mediated epitope retrieval is used as pre-treatment.  Blocking of the endogenous peroxidase is necessary to avoid unspecific background staining. 

       

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (17)

    ab106134 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab106134 は 17 報の論文で使用されています。

    • Yang L  et al. Immutol regulates CD4+Tregs, CD8+Tregs and pDCs via IDO signaling pathway to induce immune tolerance in rat heart allograft transplant. Transpl Immunol 68:101393 (2021). PubMed: 33838298
    • Meng G  et al. Targeting aerobic glycolysis by dichloroacetate improves Newcastle disease virus-mediated viro-immunotherapy in hepatocellular carcinoma. Br J Cancer 122:111-120 (2020). PubMed: 31819179
    • Fujii K  et al. Indoleamine 2,3-Dioxygenase 2 Deficiency Exacerbates Imiquimod-Induced Psoriasis-Like Skin Inflammation. Int J Mol Sci 21:N/A (2020). PubMed: 32752186
    • Guo G  et al. IDO1 depletion induces an anti-inflammatory response in macrophages in mice with chronic viral myocarditis. Cell Cycle 18:2598-2613 (2019). PubMed: 31416389
    • Jiao H  et al. Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus. Neuropsychiatr Dis Treat 15:21-31 (2019). PubMed: 30587994
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question


    I am going to test Rb anti-Ido1 ab106134.

    I know this antibody had been tested in your company and works on human, mouse and rat tissue.

    Could you let me know which tissue of mouse or rat was used for testing and detail of protocol as possible?

    Thanks for help!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    My name is Jeremy Kasanovand I'm helping Sybille with her emails while she is out of the office.

    The antibody was tested by thelab which developed this antibodyfor cross reactivity with mouse IDO in Western blot. Unfortunately we have no details which control material was used.

    As for the reactivity on rat IDO, the details as confirmed in reference is the only data available.
    Sorry I can't be more helpful in this instance. Please contact us again if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Question

    Thanks for the information!

    It is not easy to get rat breast cancer tissue for most of Lab. I am wondering besides it, is there any other rat tissue can be used to validate the antibody ab106134? Also, this antibody had been tested on mouse tissue, what is the mouse tissue should be used?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 05 2012

    Answer

    Thank you for your reply.

    The information the lab sent me is all we have available. There is also a reference listed on the datasheet where human and rat tissue was used:

    Hill M et al. Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2,3-dioxygenase. FASEB J 19:1957-68 (2005). Human, Rat. PubMed: 16319139

    I will check again regarding mouse testing.

    Also, I checkedthe human protein Atlas entry for IDO1 (http://www.proteinatlas.org/ENSG00000131203) where you will find more information about normal and cancer tissues as well as cell lines that express the protein and to which extend. For instance human lymphnodes express the protein strongly (http://www.proteinatlas.org/ENSG00000131203/normal). Human ovarian and cervical cancer tissue expresses the protein as well (http://www.proteinatlas.org/ENSG00000131203/cancer).

    We have a collection of tissue slides that you can use as positive control slides - mainly human (https://www.abcam.com/index.html?c=2300), some for mouse (https://www.abcam.com/index.html?c=2301) and also a few for rat.

    We do carry human lymph node slides (ab4350, https://www.abcam.com/Lymph-node-human-normal-tissue-slides-ab4350.html).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2012

    Question

    Thank you for your follow-up. I am interested in the offer that you have described below. Would you please send the discount code and then I will proceed with purchasing ab106134.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2012

    Answer

    I am very pleased to hear you would like to use ab106134 in mouse and send us an image of your results.

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

    If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 13 2012

    Question

    I am writing to follow-up on the status of my inquiry regarding ab106134 and what tissue was tested in mouse.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thank you very much for your interest in ab106134 and for your patience.

    Unfortunately I have not been able to obtain any more information on the tissue tested in mouse and I do not want for you to have to continue to wait for a response.


    Since we do not have an image available, I can offer a discount off a future purchase if you buy ab106134 now, use it in mouse and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

    It doesn't matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab106134 in mouse. I will then send a discount code. This code must be issued before purchasing ab103164 so please wait for my reply before ordering.

    2. Purchase ab106134 either by phone, fax, or online (www.abcam.com).

    3. Use it in mouse.

    4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated!

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 09 2012

    Question

    I sould like to test these antibody in IHC-P using tissues from pigtail macaque (monkey).

    Read More

    Abcam community

    Verified customer

    Asked on Jan 20 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test ab55305 and ab106134 in monkey. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody in monkey and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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