Recombinant Simian Virus 40 Simian Virus 40 Major Capsid VP1 protein (ab74565)
Key features and details
- Expression system: Saccharomyces cerevisiae
- Purity: > 85% SDS-PAGE
- Suitable for: ELISA, WB, SDS-PAGE
製品の詳細
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製品名
Recombinant Simian Virus 40 Simian Virus 40 Major Capsid VP1 protein -
精製度
> 85 % SDS-PAGE.
Purified by ultracentifugation -
発現系
Saccharomyces cerevisiae -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Simian Virus 40 -
配列
MKMAPTKRKGSCPGAAPKKPKEPVQVPKLVIKGGIEVLGVKTGVDSFTEV ECFLNPQMGNPDEHQKGLSKSLAAEKQFTDDSPDKEQLPCYSVARIPLPN LNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHENGAGKPIQGS NFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKAVL DKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDE QGVGPLCKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKN PYPISFLLSDLINRRTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMI RYIDEFGQTTTRLQ
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特性
Our Abpromise guarantee covers the use of ab74565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
ELISA
Western blot
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C.
Constituent: PBS
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再構成Reconstitute with deionized H2O.
関連情報
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別名
- Major capsid protein VP1
- Major structural protein VP1
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関連性
Simian virus 40 (SV40) is a small, non enveloped DNA virus with an icosahedral capsid of 45 nm. The simian virus 40 capsid is composed of 72 pentamers of VP1, the major protein of SV40. These pentamers are arranged in a T=7d icosahedral surface lattice, which is maintained by three types of appropriately arranged, non-equivalent interactions between the pentamers. Simian Virus 40 Major Capsid VP1 binds to N-glycolylneuraminic analog of the ganglioside GM1 on the cell surface to provide virion attachment to target cell. Once attached, the virion enters a caveolae and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. The assembly takes place in the cell nucleus, encapsulates the genomic DNA and participates in rearranging nucleosomes around the viral DNA. The viral progenies exit the cells by lytic release. -
細胞内局在
Virion. Nucleus. Endoplasmic reticulum. Note=Following host cell entry, the virion enters into the endoplasmic reticulum through a calveolar-dependent pathway. Then, viral DNA is translocated to the nucleus. Shortly after synthesis, a nuclear localization signal directs VP1 to the cell nucleus where virion assembly occurs.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab74565 は論文での使用が確認できていません。