Name: Anti-INCENP antibody [58-217]
SKU: ab23956
Rating: 4 (3 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)

Key features and details

Mouse monoclonal [58-217] to INCENP
Suitable for: ICC/IF, IHC-P
Reacts with: Human
Isotype: IgG1

製品名

Anti-INCENP antibody [58-217]
INCENP 一次抗体製品一覧
製品の詳細

Mouse monoclonal [58-217] to INCENP
由来種

Mouse
アプリケーション

適用あり: ICC/IF, IHC-Pmore details
種交差性

交差種: Human
非交差種: Mouse, Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- IHC-P: Human anal cancer tissue
特記事項

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Concentration information loading...
精製度

IgG fraction
ポリ/モノ

モノクローナル
クローン名

58-217
アイソタイプ

IgG1
研究分野

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
Recombinant Protein
- Recombinant Human INCENP protein (ab135223)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab23956の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF	(1)	Use a concentration of 1 µg/ml.
IHC-P	(1)	Use at an assay dependent concentration.

特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.

機能

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1.
配列類似性

Belongs to the INCENP family.
細胞内局在

Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
Target information above from: UniProt accession Q9NQS7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 3619 Human
- Omim: 604411 Human
- SwissProt: Q9NQS7 Human
- Unigene: 142179 Human
別名
- binds and activates aurora B and C in vivo and in vitro antibody
- Chromosomal passenger protein antibody
- INCE_HUMAN antibody
- INCENP antibody
- Inner centromere protein antibody
- Inner centromere protein antigens 135/155kDa antibody
- Inner centromere protein antigens 135kD 155kD antibody
- Inner centromere protein INCENP antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)

ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INCENP antibody [58-217] (ab23956)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.

ab23956 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-mouse antibody was used as the secondary.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)Image kindly submitted by Serena Orlando, Giulia Guarguaglini and Patrizia Lavia, from the University 'La Sapienza' CNR, Italy

ab23956 staining INCENP in HeLa cells, by immunofluorescence.
optimal antibody dilution: 1mg/ml
Immunocytochemistry/ Immunofluorescence - Anti-INCENP antibody [58-217] (ab23956)Image from Mackay DR et al, J Cell Biol. 2010 Nov 29;191(5):923-31. Epub 2010 Nov 22, Fig 3. DOI 10.1083/jcb.201007124

ab23956 staining INCENP in siRNA-treated HeLa cells by Immunocytochemistry/ Immunofluorescence.

Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheet download

Download

ab23956 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab23956 は 5 報の論文で使用されています。

Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
Baron AP et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. Elife 5: (2016). PubMed: 26885717
Park H et al. Ewing sarcoma EWS protein regulates midzone formation by recruiting Aurora B kinase to the midzone. Cell Cycle 13:2391-9 (2014). PubMed: 25483190
Kim HS et al. Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates sister chromatid cohesion. Nat Commun 4:2631 (2013). PubMed: 24149858
Vazquez-Martin A et al. Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 10:3140-52 (2011). ICC/IF ; Human . PubMed: 21900751

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-3 of 3 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Colon carcinoma)

Specification

Colon carcinoma

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.5% Triton in PBS

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1%

DR. Eleni Petsalaki

Verified customer

投稿 Dec 11 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa, U2OS, Jurkat)

Loading amount

20 µg

Specification

HeLa, U2OS, Jurkat

Gel Running Conditions

Reduced Denaturing (5% SDS-PAGE)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

投稿 Aug 22 2008

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (anal cancer)

Specification

anal cancer

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0

Permeabilization

Blocking step

Sequential pre-diluted peroxidase (10 min) and protein (10 min) blocks. as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C

Mr. Antibody Solutions

Verified customer

投稿 Jul 17 2008

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (5)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-INCENP antibody [58-217]

Western blot abreview for Anti-INCENP antibody [58-217]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-INCENP antibody [58-217]