Anti-INCENP 抗体 [58-217] (ab23956)
Key features and details
- Mouse monoclonal [58-217] to INCENP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-INCENP antibody [58-217]
INCENP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [58-217] to INCENP -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human anal cancer tissue
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
58-217 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23956の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Probably acts through association with AURKB or AURKC. Seems to bind directly to microtubules. Controls the kinetochore localization of BUB1. -
配列類似性
Belongs to the INCENP family. -
細胞内局在
Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Nucleus. Chromosome > centromere > kinetochore. Localizes to inner kinetochore. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 3619 Human
- Omim: 604411 Human
- SwissProt: Q9NQS7 Human
- Unigene: 142179 Human
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別名
- binds and activates aurora B and C in vivo and in vitro antibody
- Chromosomal passenger protein antibody
- INCE_HUMAN antibody
see all
画像
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ICC/IF image of ab23956 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab23956, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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ab23956 staining human anal cancer tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retreival in citrate buffer (pH 6) prior to blocking in a commercially available blocking agent for 20 minutes at 20°C. The primary anibody was diluted 1/200 and incubated with the sample for 40 minutes at 20°C. A HRP-conjugated goat anti-mouse antibody was used as the secondary.
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ab23956 staining INCENP in HeLa cells, by immunofluorescence.
optimal antibody dilution: 1mg/ml
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab23956 は 5 報の論文で使用されています。
- Murillo-Pineda M et al. Induction of spontaneous human neocentromere formation and long-term maturation. J Cell Biol 220:N/A (2021). PubMed: 33443568
- Baron AP et al. Probing the catalytic functions of Bub1 kinase using the small molecule inhibitors BAY-320 and BAY-524. Elife 5: (2016). PubMed: 26885717
- Park H et al. Ewing sarcoma EWS protein regulates midzone formation by recruiting Aurora B kinase to the midzone. Cell Cycle 13:2391-9 (2014). PubMed: 25483190
- Kim HS et al. Functional interplay between Aurora B kinase and Ssu72 phosphatase regulates sister chromatid cohesion. Nat Commun 4:2631 (2013). PubMed: 24149858
- Vazquez-Martin A et al. Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 10:3140-52 (2011). ICC/IF ; Human . PubMed: 21900751