Anti-Importin 7 抗体 (ab99273)
Key features and details
- Rabbit polyclonal to Importin 7
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Importin 7 antibody
Importin 7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Importin 7 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
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免疫原
Synthetic peptide within Human Importin 7 aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006382.1
Database link: O95373 -
ポジティブ･コントロール
- HeLa whole cell lysate (ab150035), 293T whole cell lysate and Mouse NIH3T3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab99273 was affinity purified using an epitope specific to Importin 7 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
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| IP |
Use at 5-15 µg/mg of lysate.
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| IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 120 kDa. |
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IP
Use at 5-15 µg/mg of lysate. |
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IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. -
配列類似性
Belongs to the importin beta family.
Contains 1 importin N-terminal domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 513707 Cow
- Entrez Gene: 485383 Dog
- Entrez Gene: 10527 Human
- Entrez Gene: 233726 Mouse
- Entrez Gene: 308939 Rat
- Omim: 605586 Human
- SwissProt: O95373 Human
- SwissProt: Q9EPL8 Mouse
see all -
別名
- FLJ14581 antibody
- Imp 7 antibody
- Imp7 antibody
see all
画像
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Western blot - Anti-Importin 7 antibody (ab99273)All lanes : Anti-Importin 7 antibody (ab99273) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin 7 antibody (ab99273)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Importin 7 with ab99273 at 1/1000 (0.2µg/mg). Detection: DAB. -
Immunoprecipitation - Anti-Importin 7 antibody (ab99273)ab99273 at 0.1 µg/ml detecting Importin 7 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
For immunoprecipitation, ab99273 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99272 which recognizes an upstream epitope of Importin 7.
Lane 2; IP using ab99273.
Lane 3; IP using control IgG.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab99273 は 8 報の論文で使用されています。
- Astapova O et al. Ligand Binding Prolongs Androgen Receptor Protein Half-Life by Reducing its Degradation. J Endocr Soc 5:bvab035 (2021). PubMed: 33869982
- Moriyama T et al. Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-a. Sci Rep 10:18640 (2020). PubMed: 33122699
- Helleboid PY et al. The interactome of KRAB zinc finger proteins reveals the evolutionary history of their functional diversification. EMBO J 38:e101220 (2019). PubMed: 31403225
- Hu X et al. Mitogen-activated protein kinase inhibitors reduce the nuclear accumulation of phosphorylated Smads by inhibiting Imp 7 or Imp 8 in HepG2 cells. Oncol Lett 15:4867-4872 (2018). PubMed: 29552126
- Wu C et al. Compound Astragalus and Salvia miltiorrhiza extract inhibits hepatocarcinogenesis via modulating TGF-ß/TßR and Imp7/8. Exp Ther Med 16:1052-1060 (2018). PubMed: 30112050
- Baas R et al. Quantitative Proteomics of the SMAD (Suppressor of Mothers against Decapentaplegic) Transcription Factor Family Identifies Importin 5 as a Bone Morphogenic Protein Receptor SMAD-specific Importin. J Biol Chem 291:24121-24132 (2016). PubMed: 27703004
- Putker M et al. Evolutionary acquisition of cysteines determines FOXO paralog-specific redox signaling. Antioxid Redox Signal 22:15-28 (2015). PubMed: 25069953
- Wei Y et al. Importin 8 regulates the transport of mature microRNAs into the cell nucleus. J Biol Chem 289:10270-5 (2014). Mouse . PubMed: 24596094
