Anti-IMPDH2 抗体 [EPR8365(B)] - BSA and Azide free (ab248323)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8365(B)] to IMPDH2 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IMPDH2 antibody [EPR8365(B)] - BSA and Azide free
IMPDH2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8365(B)] to IMPDH2 - BSA and Azide free -
由来種
Rabbit -
特異性
The immunogen used to raise this antibody shares 86% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.
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アプリケーション
適用あり: IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa. K-562, MCF7 whole cell lysate, Rat and Mouse spleen lysate, DDDK tagged human full length IMPDH2 recombinant protein; IHC-P: Human colon and ovarian cancer tissue; ICC/IF: HeLa cells; Flow Cyt (Intra): K-562 cells.
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特記事項
ab248323 is the carrier-free version of ab129165.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 2.24 x 10 -9 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8365(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
- PE Anti-IMPDH2 antibody [EPR8365(B)] (ab306295)
- APC Anti-IMPDH2 antibody [EPR8365(B)] (ab306296)
- HRP Anti-IMPDH2 antibody [EPR8365(B)] (ab306297)
- Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8365(B)] (ab309809)
- Alexa Fluor® 647 Anti-IMPDH2 antibody [EPR8365(B)] (ab310178)
- Alexa Fluor® 594 Anti-IMPDH2 antibody [EPR8365(B)] (ab310617)
- Alexa Fluor® 555 Anti-IMPDH2 antibody [EPR8365(B)] (ab312145)
- Alexa Fluor® 568 Anti-IMPDH2 antibody [EPR8365(B)] (ab312631)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248323の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
組織特異性
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
パスウェイ
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
配列類似性
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. -
翻訳後修飾
The N-terminus is blocked. - Information by UniProt
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参照データベース
- Entrez Gene: 3615 Human
- Entrez Gene: 23918 Mouse
- Entrez Gene: 301005 Rat
- Omim: 146691 Human
- SwissProt: P12268 Human
- SwissProt: P24547 Mouse
- SwissProt: E9PU28 Rat
- Unigene: 654400 Human
see all -
別名
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
see all
画像
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All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/10000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 60 secondsThis data was developed using ab129165, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/1000 dilution
Lane 1 : DDDK tagged human full length IMPDH1 recombinant protein
Lane 2 : DDDK tagged human full length IMPDH2 recombinant protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 140 secondsThis data was developed using ab129165, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST -
This data was developed using ab129165, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling IMPDH2 with ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
This data was developed using ab129165, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue labelling IMPDH2 with ab129165 at 1/4000 for 30 mins at room temperature. A Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. Negative control using only secondary antibody. Counterstained with hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. -
This data was developed using ab129165, the same antibody clone in a different buffer formulation.
Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labelling IMPDH2 (Green) with ab129165 at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG H&L, was used as the secondary antibody at a 1/1000. Tubulin (Red) was stained with ab195889, an Alexa Fluor® 594-conjugated anti-alpha tubulin antibody, at a 1/200 dilution. DAPI (Blue) was used as the nuclear counterstain. Negative control using only secondary antibody.
Confocal image showing cytoplasmic and weak nuclear staining in HeLa cells.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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This data was developed using ab129165, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) cells labeling IMPDH2 with ab129165 at 1/20 dilution (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% Methanol. Rabbit monoclonal - Alexa Fluor® 488 (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248323 は論文での使用が確認できていません。