Anti-IMP3 抗体 [EPR12021] (ab177477)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12021] to IMP3
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IMP3 antibody [EPR12021]
IMP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12021] to IMP3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IPmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human IMP3 aa 1-250. The exact sequence is proprietary.
Database link: O00425 -
ポジティブ・コントロール
- WB: MDA-MB-231 whole cell lysate, MDA-MB-435S whole cell lysate, HeLa whole cell lysate, HEK-293 whole cell lysate, HAP1 whole cell lysate, Mouse placenta lysate, Mouse spleen lysate, Rat spleen lysate, Rat heart lysate. IP: 293T whole cell lysate. ICC/IF: HeLa cells. Flow cyto(intra): BxPC-3 (human pancreas adenocarcinoma) cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12021 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177477の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/1000 - 1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa). |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. -
組織特異性
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. -
配列類似性
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
ドメイン
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
細胞内局在
Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. - Information by UniProt
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参照データベース
- Entrez Gene: 10643 Human
- Entrez Gene: 140488 Mouse
- Entrez Gene: 312320 Rat
- Omim: 608259 Human
- SwissProt: O00425 Human
- SwissProt: Q9CPN8 Mouse
- Unigene: 700696 Human
- Unigene: 281018 Mouse
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別名
- Cancer/testis antigen 98 antibody
- CT98 antibody
- DKFZp686F1078 antibody
see all
画像
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Immunoprecipitation of IMP3 from 293T (Human embryonic kidney epithelial cell) whole cell lysate using ab177477 at 1/20 dilution.
Lane 1: 293T (Human embryonic kidney epithelial cell) whole cell lysate 10μg
Lane 2: 293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab177477 in 293T whole cell lysate
ab131366 was used as secondary antibody at 1/1000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Observed MW at 69kDa.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling IMP3 using ab177477. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab177477 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/10000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
We are unsure about the nature of the extra bands.
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All lanes : Anti-IMP3 antibody [EPR12021] (ab177477) at 1/10000 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 5 : Mouse placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
We are unsure about the nature of the extra bands.
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab177477 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab177477 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IGF2BP3 (IMP3) knockout cells were examined. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177477 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Intracellular Flow Cytometry analysis of BxPC-3 (human pancreas adenocarcinoma) cells labeling with purified ab177477 at 1/300 dilution (10ug/ml) (Red). Cells were fixed with 80% methanoland permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab177477 は 11 報の論文で使用されています。
- Cui J et al. Comprehensive analysis of N6-methyladenosine regulators with the tumor immune landscape and correlation between the insulin-like growth factor 2 mRNA-binding protein 3 and programmed death ligand 1 in bladder cancer. Cancer Cell Int 22:72 (2022). PubMed: 35148766
- Wang C et al. IGF2BP3 enhances the mRNA stability of E2F3 by interacting with LINC00958 to promote endometrial carcinoma progression. Cell Death Discov 8:279 (2022). PubMed: 35676262
- Li B et al. The N6-methyladenosine-mediated lncRNA WEE2-AS1 promotes glioblastoma progression by stabilizing RPN2. Theranostics 12:6363-6379 (2022). PubMed: 36168628
- Wang X et al. RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent. J Exp Clin Cancer Res 40:80 (2021). PubMed: 33637103
- Zhu J & Han S Downregulation of LncRNA DARS-AS1 Inhibits the Tumorigenesis of Cervical Cancer via Inhibition of IGF2BP3. Onco Targets Ther 14:1331-1340 (2021). PubMed: 33658798