Anti-ILF1 抗体 (ab5298)
Key features and details
- Goat polyclonal to ILF1
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ILF1 antibody
ILF1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ILF1 -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: TPPAAVREKGVQN, corresponding to C terminal amino acids 648-660 of Human ILF1.
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ポジティブ･コントロール
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa). A 1 hour primary incubation is recommended for this product. |
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| Flow Cyt | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements. -
組織特異性
Expressed in both lymphoid and non-lymphoid cells. -
配列類似性
Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3607 Human
- Omim: 147685 Human
- SwissProt: Q01167 Human
- Unigene: 591140 Human
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別名
- Cellular transcription factor ILF 1 antibody
- Cellular transcription factor ILF-1 antibody
- Cellular transcription factor ILF1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Flow Cytometry - Anti-ILF1 antibody (ab5298)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labeling ILF1 with ab5298. Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-ILF1 antibody (ab5298)All lanes : Anti-ILF1 antibody (ab5298) at 0.03 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) nuclear cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)Immunofluorescent analysis of paraformaldehyde fixed U2OS cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献 (6)
ab5298 は 6 報の論文で使用されています。
- Nestal de Moraes G et al. SUMOylation modulates FOXK2-mediated paclitaxel sensitivity in breast cancer cells. Oncogenesis 7:29 (2018). PubMed: 29540677
- Zhang F et al. FOXK2 suppresses the malignant phenotype and induces apoptosis through inhibition of EGFR in clear-cell renal cell carcinoma. Int J Cancer 142:2543-2557 (2018). PubMed: 29368368
- Liu Y et al. FOXK2 transcription factor suppresses ERa-positive breast cancer cell growth through down-regulating the stability of ERa via mechanism involving BRCA1/BARD1. Sci Rep 5:8796 (2015). PubMed: 25740706
- Ji Z et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res 42:6232-42 (2014). IP . PubMed: 24748658
- Ji Z et al. The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation. Mol Cell Biol 32:385-98 (2012). ChIP, WB ; Human . PubMed: 22083952
- Marais A et al. Cell cycle-dependent regulation of the forkhead transcription factor FOXK2 by CDK·cyclin complexes. J Biol Chem 285:35728-39 (2010). WB, ICC/IF ; Human . PubMed: 20810654
