Anti-ILF1 抗体 (ab5298)
Key features and details
- Goat polyclonal to ILF1
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ILF1 antibody
ILF1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ILF1 -
由来種
Goat -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: TPPAAVREKGVQN, corresponding to C terminal amino acids 648-660 of Human ILF1.
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ポジティブ･コントロール
- ウェスタン・ブロット用ポジティブコントロールRecombinant Human ILF1 protein
- Flow Cyt (Intra): HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5298の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB |
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 75-80 kDa (predicted molecular weight: 73 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements. -
組織特異性
Expressed in both lymphoid and non-lymphoid cells. -
配列類似性
Contains 1 FHA domain.
Contains 1 fork-head DNA-binding domain. -
ドメイン
The C-terminal part of the DNA-binding domain may contribute to DNA recognition specificity. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3607 Human
- Omim: 147685 Human
- SwissProt: Q01167 Human
- Unigene: 591140 Human
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別名
- Cellular transcription factor ILF 1 antibody
- Cellular transcription factor ILF-1 antibody
- Cellular transcription factor ILF1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Flow Cytometry (Intracellular) - Anti-ILF1 antibody (ab5298)Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labeling ILF1 with ab5298. Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-ILF1 antibody (ab5298)All lanes : Anti-ILF1 antibody (ab5298) at 0.03 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) nuclear cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) nuclear cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 73 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)Immunofluorescent analysis of paraformaldehyde fixed U2OS cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
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Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab5298 は 7 報の論文で使用されています。
- Xia YK et al. Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network. Protein Cell 12:557-577 (2021). PubMed: 32683582
- Nestal de Moraes G et al. SUMOylation modulates FOXK2-mediated paclitaxel sensitivity in breast cancer cells. Oncogenesis 7:29 (2018). PubMed: 29540677
- Zhang F et al. FOXK2 suppresses the malignant phenotype and induces apoptosis through inhibition of EGFR in clear-cell renal cell carcinoma. Int J Cancer 142:2543-2557 (2018). PubMed: 29368368
- Liu Y et al. FOXK2 transcription factor suppresses ERa-positive breast cancer cell growth through down-regulating the stability of ERa via mechanism involving BRCA1/BARD1. Sci Rep 5:8796 (2015). PubMed: 25740706
- Ji Z et al. The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex. Nucleic Acids Res 42:6232-42 (2014). IP . PubMed: 24748658
- Ji Z et al. The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation. Mol Cell Biol 32:385-98 (2012). ChIP, WB ; Human . PubMed: 22083952
- Marais A et al. Cell cycle-dependent regulation of the forkhead transcription factor FOXK2 by CDK·cyclin complexes. J Biol Chem 285:35728-39 (2010). WB, ICC/IF ; Human . PubMed: 20810654
