リコンビナントRabMAb

Anti-IL3RA/CD123 抗体 [EPR23188-72] - BSA and Azide free (ab269871)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
  • ELISA - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
  • Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23188-72] to IL3RA/CD123 - BSA and Azide free
  • Suitable for: ELISA, WB, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free
    IL3RA/CD123 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23188-72] to IL3RA/CD123 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HDLM-2, TF-1, Human tonsil and Human tonsil treated with PNGase F lysates. IHC-P: Human tonsil tissue.

  • 特記事項

    ab269871 is the carrier-free version of ab222103. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ELISA
Recombinant fragment
IHC-P
Human
アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60, 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 60, 43 kDa (predicted molecular weight: 43 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for ICC/IF,IHC-Fr or IP.

    • ターゲット情報

    • 機能

      This is a receptor for interleukin-3.

    • 配列類似性

      Belongs to the type I cytokine receptor family. Type 5 subfamily.

    • ドメイン

      The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
      The box 1 motif is required for JAK interaction and/or activation.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD123 antibody
        • CD123 antigen antibody
        • hIL 3Ra antibody
        • hIL-3Ra antibody
        • hIL3Ra antibody
        • IL 3 receptor alpha SP2 isoform antibody
        • IL 3R alpha antibody
        • IL-3 receptor subunit alpha antibody
        • IL-3R subunit alpha antibody
        • IL-3R-alpha antibody
        • IL-3RA antibody
        • IL3 Receptor alpha antibody
        • IL3R antibody
        • Il3ra antibody
        • IL3RA_HUMAN antibody
        • IL3RAX antibody
        • IL3RAY antibody
        • IL3RX antibody
        • IL3RY antibody
        • Interleukin 3 receptor alpha chain antibody
        • Interleukin 3 receptor, alpha (low affinity) antibody
        • Interleukin 3 receptor, alpha antibody
        • Interleukin-3 Receptor alpha antibody
        • Interleukin-3 receptor subunit alpha antibody
        • Interleukin3 receptor antibody
        • Interleukin3 receptor, Y-chromosomal antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IL3RA/CD123 with ab222103 at 1/500 dilution (0.99 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 27340692). The section was incubated with ab222103 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222103).

      • ELISA - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
        ELISA - Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)

        This data was developed using ab222103, the same antibody clone in a different buffer formulation.

        ELISA analysis of Human IL3RA/CD123 recombinant protein at 1000 ng/mL with ab222103. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
      • Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)
        Anti-IL3RA/CD123 antibody [EPR23188-72] - BSA and Azide free (ab269871)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269871 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269871 は論文での使用が確認できていません。

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