Anti-IL36 gamma/IL-1F9 抗体 [EPR23333-107] (ab272731)
![Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.co.jp/ps/products/272/ab272731/Images/ab272731-392016-anti-il36-gammail1f9-antibody-epr23333107-western-blot-hacat.jpg "Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9
- Suitable for: WB
- Reacts with: Human
製品の概要
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製品名
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107]
IL36 gamma/IL-1F9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23333-107] to IL36 gamma/IL-1F9 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HaCaT (treated with 20ng/ml IL-1beta and 50ng/ml TNFalpha for 20 hours,) whole cell lysate; His-tagged recombinant human IL36G protein (aa18-169).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23333-107 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| IHC-P |
Mouse
Rat
Human
|
| IP |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 19 kDa). |
ターゲット情報
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機能
Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response. -
組織特異性
Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin. -
配列類似性
Belongs to the IL-1 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 56300 Human
- Omim: 605542 Human
- SwissProt: Q9NZH8 Human
- Unigene: 211238 Human
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別名
- IL 1 epsilon antibody
- IL 1 related protein 2 antibody
- IL 1(EPSILON) antibody
see all
画像
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Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)All lanes : Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution
Lane 1 : HaCaT (human skin keratinocyte), whole cell lysate
Lane 2 : HaCaT treated with 20ng/ml IL-1beta and 50ng/ml TNF-alpha for 20 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of IL36 gamma/IL-1F9 is induced by IL-1beta and TNF-alpha treatment in HaCaT (PMID: 23095752).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
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![Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.co.jp/ps/products/272/ab272731/Images/ab272731-392017-anti-il36-gammail1f9-antibody-epr23333107-western-blot-histagged-recombinant-human-il36g-protein.jpg)
Western blot - Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731) at 1/1000 dilution + His-tagged recombinant human IL36G protein (aa18-169), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 19 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
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![Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)](https://www.abcam.co.jp/ps/products/272/ab272731/Images/ab272731-3-benefits-of-recombinant-antibodies.png)
Anti-IL36 gamma/IL-1F9 antibody [EPR23333-107] (ab272731)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
Certificate of Compliance
参考文献 (0)
ab272731 は論文での使用が確認できていません。
