PE Anti-IL-13 抗体 [13A] (ab95576)
Key features and details
- PE Rat monoclonal [13A] to IL-13
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG1
製品の概要
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製品名
PE Anti-IL-13 antibody [13A]
IL-13 一次抗体 製品一覧 -
製品の詳細
PE Rat monoclonal [13A] to IL-13 -
由来種
Rat -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant full length protein corresponding to Mouse IL-13.
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ポジティブ・コントロール
- BALB/c splenocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
13A -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 0.25µg for 105-8 cells.
ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 0.25µg for 105-8 cells. ab154449 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
関連疾患
Allergic rhinitis -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16163 Mouse
- SwissProt: P20109 Mouse
- Unigene: 1284 Mouse
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別名
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
see all
画像
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ab95576 at 0.125 µg/test staining IL-13 by Flow Cytometry.
BALB/c splenocytes were cultured for 3 days with plate-bound Anti-Mouse CD3e, soluble Anti-Mouse CD28, Mouse IL2 Recombinant Protein and Mouse IL4 Recombinant Protein. Cells were then harvested and restimulated with PMA and Ionomycin in the presence of monensin for 5 hours. Following restimulation, cells were harvested, surface stained with Anti-Mouse CD4 FITC, fixed and permeabilized, then stained with 0.125 µg of ab95576 (1). The ligand blocking control demonstrates that pre-blocking ab95576 with 1.0 µg of Mouse IL-13 Recombinant Protein prior to staining abrogates specific mouse IL-13 staining (2).
データシートおよび資料
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Datasheet download
参考文献 (1)
ab95576 は 1 報の論文で使用されています。
- Poe C et al. Treatment of established TH2 cells with 4µ8c, an inhibitor of IRE1a, blocks IL-5 but not IL-4 secretion. BMC Immunol 20:3 (2019). PubMed: 30630412