Anti-IL-1 alpha 抗体 (ab9614)
Key features and details
- Rabbit polyclonal to IL-1 alpha
- Suitable for: ICC/IF, Sandwich ELISA, WB, Neutralising, IHC-P, ELISA
- Reacts with: Human
- Isotype: unknown
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-1 alpha antibody
IL-1 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-1 alpha -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, Sandwich ELISA, WB, Neutralising, IHC-P, ELISAmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human IL-1 alpha. Highly pure (>98%) recombinant hIL-1a (human Interleukin-1-alpha).
Database link: P01583 -
ポジティブ・コントロール
- IHC-P: Human liver tissue; Recombinant human IL-1 alpha protein (ab9615) can be used as a positive control in WB.
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特記事項
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab7632
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
No preservative, sterile filtered -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9614の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
|
Sandwich ELISA |
Use at an assay dependent concentration.
To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
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WB | (1) |
Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ELISA |
Use at an assay dependent concentration.
To detect hIL-1a by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. To detect IL-1 αlpha by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab271226 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant IL-1 αlpha. |
WB
Use at an assay dependent concentration. To detect hIL-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-1a (20 ng/ml), a concentration of 1.0 - 2.0 µg/ml of this antibody is required. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ELISA
Use at an assay dependent concentration. To detect hIL-1a by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-1a. |
ターゲット情報
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機能
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
配列類似性
Belongs to the IL-1 family. -
ドメイン
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
細胞内局在
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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参照データベース
- Entrez Gene: 3552 Human
- Omim: 147760 Human
- SwissProt: P01583 Human
- Unigene: 1722 Human
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別名
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling IL-1 alpha with ab9614 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab9614 は 19 報の論文で使用されています。
- Khodadadei F et al. The effect of A1 and A2 reactive astrocyte expression on hydrocephalus shunt failure. Fluids Barriers CNS 19:78 (2022). PubMed: 36171630
- Schunk SJ et al. Interleukin-1α Is a Central Regulator of Leukocyte-Endothelial Adhesion in Myocardial Infarction and in Chronic Kidney Disease. Circulation 144:893-908 (2021). PubMed: 34192892
- Tanaka Y et al. Baicalein Inhibits Benzo[a]pyrene-Induced Toxic Response by Downregulating Src Phosphorylation and by Upregulating NRF2-HMOX1 System. Antioxidants (Basel) 9:N/A (2020). PubMed: 32526964
- Guttenplan KA et al. Neurotoxic Reactive Astrocytes Drive Neuronal Death after Retinal Injury. Cell Rep 31:107776 (2020). PubMed: 32579912
- Moreno-Valladares M et al. CD8+ T cells are increased in the subventricular zone with physiological and pathological aging. Aging Cell 19:e13198 (2020). PubMed: 32741087