Anti-IL-9 抗体 [EPR23484-151] (ab227037)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23484-151] to IL-9
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-9 antibody [EPR23484-151]
IL-9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23484-151] to IL-9 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IF, WB, IPmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged mouse IL9 recombinant protein, Mouse and rat spleen, thymus lysates. ICC/IF: 293T cell. Flow Cyt (intra): 293T cell. IP: Mouse spleen lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23484-151 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227037の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
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ICC/IF |
1/500.
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|
WB |
1/1000. Predicted molecular weight: 15 kDa.
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|
IP |
1/30.
|
特記事項 |
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Flow Cyt (Intra)
1/500. |
ICC/IF
1/500. |
WB
1/1000. Predicted molecular weight: 15 kDa. |
IP
1/30. |
ターゲット情報
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機能
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
配列類似性
Belongs to the IL-7/IL-9 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3578 Human
- Entrez Gene: 16198 Mouse
- Entrez Gene: 116558 Rat
- Omim: 146931 Human
- SwissProt: P15248 Human
- SwissProt: P15247 Mouse
- Unigene: 960 Human
- Unigene: 3006 Mouse
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別名
- Cytokine P40 antibody
- Homolog of mouse T cell and mast cell growth factor 40 antibody
- HP40 antibody
see all
画像
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All lanes : Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution
Lane 1 : Rat spleen tissue lysate
Lane 2 : Rat thymus tissue lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).
Exposure time: 104 seconds.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10µg with ab227037 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10µg
Lane 2: ab227037 IP in mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227037 in mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
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All lanes : Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).
Exposure time: 3 minutes.
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Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution + His-tagged mouse IL9 recombinant protein, 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 15 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).
Exposure time: 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab227037 は論文での使用が確認できていません。