Anti-IL-6 抗体 [EPR16610-69] (ab179570)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16610-69] to IL-6
- Suitable for: ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-6 antibody [EPR16610-69]
IL-6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16610-69] to IL-6 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: RAW 264.7 cells. IP: RAW 264.7 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16610-69 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179570の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
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IP |
1/30.
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特記事項 |
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ICC/IF
1/100. |
IP
1/30. |
ターゲット情報
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
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別名
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
画像
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IL-6 was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179570 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179570 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: RAW 264.7 treated with 100ng/ml lipopolysaccharides (LPS) for 3h, then together with 300ng/ml BFA for another 3h whole cell lysate, 10 µg (Input).
Lane 2: ab179570 IP in RAW 264.7 treated with 100ng/ml lipopolysaccharides (LPS) for 3h, then together with 300ng/ml BFA for another 3h whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179570 in RAW 264.7 treated with 100ng/ml lipopolysaccharides (LPS) for 3h, then together with 300ng/ml BFA for another 3h whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling IL-6 with ab179570 at 1/100 dilution, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing an elevated cytoplasmic staining in RAW 264.7 cells treated with LPS (100 ng/ml, 6 h) then together with 300 ng/ml BFA for another 3h. The nuclear counter stain is DAPI (blue). Tubuin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (5)
ab179570 は 5 報の論文で使用されています。
- Gao P et al. circEXOC5 promotes acute lung injury through the PTBP1/Skp2/Runx2 axis to activate autophagy. Life Sci Alliance 6:N/A (2023). PubMed: 36302650
- Xu N et al. Adiponectin Ameliorates GMH-Induced Brain Injury by Regulating Microglia M1/M2 Polarization Via AdipoR1/APPL1/AMPK/PPARγ Signaling Pathway in Neonatal Rats. Front Immunol 13:873382 (2022). PubMed: 35720361
- Chavkin NW et al. The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation. J Am Heart Assoc 10:e019904 (2021). PubMed: 34155901
- An W et al. Mechanisms of Rhizoma Coptidis against type 2 diabetes mellitus explored by network pharmacology combined with molecular docking and experimental validation. Sci Rep 11:20849 (2021). PubMed: 34675276
- Qi Y et al. A thermoreversible antibacterial zeolite-based nanoparticles loaded hydrogel promotes diabetic wound healing via detrimental factor neutralization and ROS scavenging. J Nanobiotechnology 19:414 (2021). PubMed: 34895257