Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse tumor tissue)
Permeabilization
Yes - PBST(0.5% v/v triton X-100, sigma)
Specification
mouse tumor tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody diluent (GBI Labs)
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: AF594 anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 29 2019