Application
Western blot
Sample
Human Cell lysate - whole cell (MG-63 osteosarcoma cell line)
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine gel)
Loading amount
50 µg
Treatment
LPS 10µg/mL
Specification
MG-63 osteosarcoma cell line
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
40 second(s)
Bands
Specific: around 30 kDa
DR. Joanna Plastira
Verified customer
投稿 Oct 29 2018