Application
Western blot
Sample
Mouse Cell lysate - whole cell (HL-1 mouse cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
30 µg
Treatment
LPS 10µg/mL
Specification
HL-1 mouse cardiomyocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: around 30 kDa
Additional data
Abcam response
Thank you for your honest feedback. Please note this product has low homology with the mouse sequence of IL6 (41%) and we therefore cannot guarantee the reactivity in this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Joanna Plastira
Verified customer
投稿 Oct 29 2018