Application
Western blot
Sample
Human Tissue lysate - whole (skin-biopsy)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-20%)
Loading amount
15 µg
Specification
skin-biopsy
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescence
Negative control
lane1: healthy skin
lane2: skin with psoriasis
lane 3. skin with atopic dermatitis
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 20 + 23 kDa
Positive control
none
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 30 2020