Anti-IL-4 抗体 (ab9811)
Key features and details
- Rabbit polyclonal to IL-4
- Suitable for: Sandwich ELISA, WB, ELISA, IHC-P
- Reacts with: Rat
- Isotype: unknown
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-4 antibody
IL-4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-4 -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISA, WB, ELISA, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
Recombinant full length protein corresponding to Rat IL-4. Highly pure (>98%) recombinant rIL-4 (rat Interleukin-4)
Database link: P20096 -
ポジティブ･コントロール
- WB: Recombinant rat IL4 protein (ab9812).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 - 2 µg/ml.
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| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. PubMed: 22315633
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| 特記事項 |
|---|
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Sandwich ELISA
Use a concentration of 0.5 - 2 µg/ml. |
|
WB
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 22315633 |
ターゲット情報
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機能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
関連疾患
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 287287 Rat
- SwissProt: P20096 Rat
- Unigene: 108255 Rat
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別名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
画像
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Sandwich ELISA - Anti-IL-4 antibody (ab9811)
To detect rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84278 is required. Using this antibody in conjunction with an anti-rat IL-4 (ab9811) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat IL-4.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (14)
ab9811 は 14 報の論文で使用されています。
- Wei Y et al. Exposure to ozone impacted Th1/Th2 imbalance of CD4+ T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling. Aging (Albany NY) 12:25229-25255 (2020). PubMed: 33223504
- Yang J et al. The influence of PM2.5 on lung injury and cytokines in mice. Exp Ther Med 18:2503-2511 (2019). PubMed: 31572502
- Bi CS et al. Calcitriol suppresses lipopolysaccharide-induced alveolar bone damage in rats by regulating T helper cell subset polarization. J Periodontal Res 54:612-623 (2019). PubMed: 31095745
- Chen Z et al. Interleukin-33 reduces neuronal damage and white matter injury via selective microglia M2 polarization after intracerebral hemorrhage in rats. Brain Res Bull 150:127-135 (2019). PubMed: 31129170
- Zhan T et al. Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice. Immunology 158:296-303 (2019). PubMed: 31436861
