Anti-IL-28A + IL-28B 抗体 [EPR5328(2)] - BSA and Azide free (ab248141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B - BSA and Azide free
- Suitable for: WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-28A + IL-28B antibody [EPR5328(2)] - BSA and Azide free -
製品の詳細
Rabbit monoclonal [EPR5328(2)] to IL-28A + IL-28B - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human IL-28A and IL-28B recombinant protein
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特記事項
ab248141 is the carrier-free version of ab126713.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5328(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 22 kDa. |
ターゲット情報
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細胞内局在
IL-28A: Secreted. IL-28B: Secreted. -
参照データベース
- Entrez Gene: 282616 Human
- Entrez Gene: 282617 Human
- Omim: 607401 Human
- Omim: 607402 Human
- SwissProt: Q8IZI9 Human
- SwissProt: Q8IZJ0 Human
- Unigene: 406744 Human
- Unigene: 567792 Human
画像
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All lanes : Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/1000 dilution
Lane 1 : Human IL-28A recombinant protein (aa 27 to 200)
Lane 2 : House made His-tagged full length human IL-28B recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 22 kDaExposure time
Lane 1: 40 seconds
Lane 2: 3 second
Buffer and concentration: 5% NFDM /TBST
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Anti-IL-28A + IL-28B antibody [EPR5328(2)] (ab126713) at 1/5000 dilution + Human IL-28A (amino acids 27-200) recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Exposure time: 120 secondsThis data was developed using ab126713, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248141 は論文での使用が確認できていません。