Anti-IL-25 抗体 [EPR5743(2)] - BSA and Azide free (ab250239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5743(2)] to IL-25 - BSA and Azide free
- Suitable for: WB
- Reacts with: Recombinant fragment
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-25 antibody [EPR5743(2)] - BSA and Azide free
IL-25 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5743(2)] to IL-25 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab250239 is the carrier-free version of ab180594.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5743(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab250239の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 20 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 16 kDa (predicted molecular weight: 20 kDa). |
ターゲット情報
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機能
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. -
組織特異性
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. -
配列類似性
Belongs to the IL-17 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 64806 Human
- Omim: 605658 Human
- SwissProt: Q9H293 Human
- Unigene: 302036 Human
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別名
- IL 17E antibody
- IL 25 antibody
- IL-17E antibody
see all
画像
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Anti-IL-25 antibody [EPR5743(2)] (ab180594) at 1/5000 dilution + Human IL-17E recombinant protein aa1-145 at 0.05 µg
Secondary
Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?This data was developed using ab180594, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab250239 は論文での使用が確認できていません。