Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole Lung Homogenate, RIPA-extracted protein)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Whole Lung Homogenate, RIPA-extracted protein
Blocking step
TBS Odyssey Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
2 hour(s) and 45 minute(s) · Temperature: 23°C · Diluent: 50% Odyssey TBS Blocking Buffer/Tween
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-infrared
Negative control
Omitted primary antibody (not shown on image).
Exposure
5 minute(s) and 0 second(s)
Positive control
We tested a Beta-actin antibody known to work in this system, using the same reagents and settings.
Additional data
Additional Notes
Notice that this antibody was tested with 2 different blockers and different protein amounts loaded per lane: 1) Odyssey TBS Block, and 2) Odyssey Casein block. Neither produced any bands. The attached image is #1, since only one image could be attached to this report. In both cases membranes were scanned with very high intensity laser and high quality setting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Pete Serbedzija
Verified customer
投稿 Apr 11 2016