Anti-IL-21R 抗体 (ab5980)
Key features and details
- Rabbit polyclonal to IL-21R
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-21R antibody
IL-21R 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-21R -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human IL-21R aa 97-111.
Sequence:NITDQSGNYSQECGS
(Peptide available asab6225) -
ポジティブ･コントロール
- WB: HepG2 cell lysate. IHC-P: Rat lung tissue. ICC/IF: A431 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for IL-21 function. IL-21 and its receptor activate the JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa. |
ターゲット情報
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機能
This is a receptor for interleukin-21. -
組織特異性
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen. -
配列類似性
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 50615 Human
- Entrez Gene: 60504 Mouse
- Entrez Gene: 308977 Rat
- Omim: 605383 Human
- SwissProt: Q9HBE5 Human
- SwissProt: Q9JHX3 Mouse
- Unigene: 210546 Human
- Unigene: 155643 Mouse
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別名
- CD360 antibody
- IL 21R antibody
- IL-21 receptor antibody
see all
画像
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Western blot - Anti-IL-21R antibody (ab5980)Lane 1 : Anti-IL-21R antibody (ab5980) at 1 µg/ml
Lane 2 : Anti-IL-21R antibody (ab5980) at 2 µg/ml
All lanes : HepG2 cell lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21R antibody (ab5980)ab5980 at 10µg/ml staining IL-21R in rat lung by IHC.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-21R antibody (ab5980)Immunocytochemistry/Immunofluorescence labelling A431 cells with ab5980 at 20µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-21R antibody (ab5980)Immunofluorescence of IL-21 Receptor in Rat Lung cells using ab5980 at 20 ug/ml.
プロトコール
データシートおよび資料
参考文献 (5)
ab5980 は 5 報の論文で使用されています。
- Yan L et al. IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer. Int J Oncol 54:7-16 (2019). PubMed: 30387833
- Xue D et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
- Zhang LL et al. Genome profiling revealed the activation of IL2RG/JAK3/STAT5 in peripheral T-cell lymphoma expressing the ITK-SYK fusion gene. Int J Oncol 55:1077-1089 (2019). PubMed: 31545408
- Xing R et al. Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL. Int J Mol Med 38:1125-34 (2016). PubMed: 27599586
- Wang T et al. Endothelial interleukin-21 receptor up-regulation in peripheral artery disease. Vasc Med 21:99-104 (2016). PubMed: 26705256
