Anti-IL-2 Receptor alpha 抗体 [SP176] (ab231441)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP176] to IL-2 Receptor alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-2 Receptor alpha antibody [SP176]
IL-2 Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP176] to IL-2 Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: MOLT-4, HDLM-2 and WEHI-231 whole cell lysate. IHC-P: Human endometrium and tonsil tissue. ICC/IF: HDLM-2 cells
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP176 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231441の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200. Predicted molecular weight: 31 kDa.
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IHC-P | (2) |
1/100.
Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF | (1) |
1/50.
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特記事項 |
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WB
1/200. Predicted molecular weight: 31 kDa. |
IHC-P
1/100. Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF
1/50. |
ターゲット情報
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機能
Receptor for interleukin-2. -
関連疾患
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
配列類似性
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3559 Human
- Entrez Gene: 16184 Mouse
- Entrez Gene: 25704 Rat
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P01590 Mouse
- SwissProt: P26897 Rat
- Unigene: 231367 Human
see all -
別名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
画像
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Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5µg/mL). Cells were fixed in 100% methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab231441 は 5 報の論文で使用されています。
- Zhou Y et al. Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling. Front Immunol 13:859581 (2022). PubMed: 35795662
- Tian Q et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822
- Lee JH et al. Cysteine-specific 89Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas. Front Immunol 13:1017132 (2022). PubMed: 36591250
- Cao Q et al. miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ. Ann Transl Med 9:1410 (2021). PubMed: 34733962
- Tillman H et al. Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice. Vet Pathol 57:160-171 (2020). PubMed: 31736441