Anti-IL-2 Receptor alpha 抗体 [IL2R.1] (ab9496)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)](https://www.abcam.co.jp/ps/products/9/ab9496/Images/ab9496-3-AntiIL2ReceptoralphaantibodyIL2R-1immunohistochemistrytonsilhuman.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)")
Key features and details
- Mouse monoclonal [IL2R.1] to IL-2 Receptor alpha
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-IL-2 Receptor alpha antibody [IL2R.1]
IL-2 Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IL2R.1] to IL-2 Receptor alpha -
由来種
Mouse -
特異性
This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages. -
アプリケーション
適用あり: Flow Cyt, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human IL-2 Receptor alpha.
Database link: P01589 -
ポジティブ･コントロール
- Tonsil.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3 -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
IL2R.1 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9496の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
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| IHC-P | (1) |
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
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IHC-P
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Receptor for interleukin-2. -
関連疾患
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
配列類似性
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
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別名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)Formalin-fixed, paraffin-embeded human tonsil tissue stained for IL-2 Receptor alpha using ab9496 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)](https://www.abcam.co.jp/ps/products/9/ab9496/Images/ab9496_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)ab9496 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)](https://www.abcam.co.jp/ps/products/9/ab9496/Images/ab9496-17190-anti-il-2-receptor-alpha-antibody-il2r1-flow-cytometry.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)Overlay histogram showing Jurkat cells stained with ab9496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9496, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab9496 は 2 報の論文で使用されています。
- King JY et al. Pathway analysis of coronary atherosclerosis. Physiol Genomics 23:103-18 (2005). IHC-Fr ; Human . PubMed: 15942018
- Boyd JH et al. Upper airway muscle inflammation and denervation changes in obstructive sleep apnea. Am J Respir Crit Care Med 170:541-6 (2004). PubMed: 15151922
