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  1. Link

    il-2-receptor-alpha-antibody-il2r1-ab9496.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-IL-2 Receptor alpha 抗体 [IL2R.1] (ab9496)

  • Datasheet
Reviews (1)Q&A (3)References (2)

Product price, shipping and contact information

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
  • Flow Cytometry - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)

Key features and details

  • Mouse monoclonal [IL2R.1] to IL-2 Receptor alpha
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
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Anti-CD4 antibody [EPR6855] (ab133616)
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Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (ab196372)
タンパク質
Product image
Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)

関連製品

製品の概要

  • 製品名

    Anti-IL-2 Receptor alpha antibody [IL2R.1]
    IL-2 Receptor alpha 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [IL2R.1] to IL-2 Receptor alpha
  • 由来種

    Mouse
  • 特異性

    This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
  • アプリケーション

    適用あり: Flow Cyt, IHC-Pmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human IL-2 Receptor alpha.
    Database link: P01589

  • ポジティブ・コントロール

    • Tonsil.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.3
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    IL2R.1
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    kappa
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Neuroscience
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    • Neurodegenerative disease
    • Other
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human IL-2 Receptor alpha protein (Active) (ab221397)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9496の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P (1)
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
Flow Cyt
1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Receptor for interleukin-2.
  • 関連疾患

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • 配列類似性

    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P01589 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3559 Human
    • Omim: 147730 Human
    • SwissProt: P01589 Human
    • Unigene: 231367 Human
    • 別名

      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2 Receptor alpha antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
      Formalin-fixed, paraffin-embeded human tonsil tissue stained for IL-2 Receptor alpha using ab9496 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)

      ab9496 - immunohistochemistry

      Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.

    • Flow Cytometry - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
      Flow Cytometry - Anti-IL-2 Receptor alpha antibody [IL2R.1] (ab9496)
      Overlay histogram showing Jurkat cells stained with ab9496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9496, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (2)

    ab9496 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9496 は 2 報の論文で使用されています。

    • King JY  et al. Pathway analysis of coronary atherosclerosis. Physiol Genomics 23:103-18 (2005). IHC-Fr ; Human . PubMed: 15942018
    • Boyd JH  et al. Upper airway muscle inflammation and denervation changes in obstructive sleep apnea. Am J Respir Crit Care Med 170:541-6 (2004). PubMed: 15151922

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-2 Receptor alpha antibody [IL2R.1]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Malignant Melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: High pH
    Permeabilization
    No
    Specification
    Malignant Melanoma
    Blocking step
    Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 06 2015

    Question

    We received our order this morning but the blue ice was not cold. It was room temperature. In your experience has any other customers have this problem. Also should I be concerned that it was at room temperature and at 4C?

    Thanks for your help.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 29 2012

    Answer

    Thank you for contacting Abcam.

    I am sorry that the vial you received was not kept cold. However, we do have data that provides supporting evidence that the antibody should still function properly. I have attached that data.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Nov 29 2012

    Question

    Your antibody is listed as supporting IHC-P. What antigen retreival/amplification is required.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2002

    Answer

    Use heat mediated antigen retrieval via pressure cooker or microwave. Please see the antigen retrieval protocol linked on the right hand side of the Abcam homepage. Use an ABC amplification system (also linked on the Abcam homepage).

    Read More

    Abcam Scientific Support

    Answered on Aug 09 2002

    Question

    Hi, I'm interested in using this ab as a marker for activated t cells in formalin fixed/parafiin embedded cells, frozen sections and also to label PBMC's for flow cytometry. I see that the first two on my list are specified, but has it been used for flow? Thanks a lot

    Read More

    Abcam community

    Verified customer

    Asked on Jul 15 2002

    Answer

    This antibody has never been tested in flow cytometry, therefore we can't guarantee results. However, being as this antibody recognises a cell surface marker, I would assume it would work in flow but as previously stated I can't be certain.

    Read More

    Abcam Scientific Support

    Answered on Jul 16 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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