Anti-IL-18BP 抗体 [EP1088Y] (ab52914)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1088Y] to IL-18BP
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-18BP antibody [EP1088Y]
IL-18BP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1088Y] to IL-18BP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide within Human IL-18BP aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O95998 -
ポジティブ・コントロール
- WB: HeLa and K562 cell lysates and rat kidney and heart tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1088Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52914の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 22 kDa.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 22 kDa. |
ターゲット情報
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機能
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. -
組織特異性
Strongly expressed in heart, lung, placenta and spleen. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 10068 Human
- Entrez Gene: 84388 Rat
- Omim: 604113 Human
- SwissProt: O95998 Human
- Unigene: 591967 Human
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別名
- I18BP_HUMAN antibody
- IL-18BP antibody
- IL18 binding protein antibody
see all
画像
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All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/2000 dilution (purified)
Lane 1 : Rat kidney tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 1% BSA/TBST.
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All lanes : Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/1000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : K562 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 1% BSA/TBST.
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Anti-IL-18BP antibody [EP1088Y] (ab52914) at 1/500 dilution (unpurified) + HeLa cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab52914 は 9 報の論文で使用されています。
- Meng Q et al. IL-18BP Improves Early Graft Function and Survival in Lewis-Brown Norway Rat Orthotopic Liver Transplantation Model. Biomolecules 12:N/A (2022). PubMed: 36551229
- Nakanishi Y et al. IL-18 binding protein can be a prognostic biomarker for idiopathic pulmonary fibrosis. PLoS One 16:e0252594 (2021). PubMed: 34086758
- Zhou T et al. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature 583:609-614 (2020). PubMed: 32581358
- Huang C et al. Chromatin Accessibility Regulates Gene Expression and Correlates With Tumor-Infiltrating Immune Cells in Gastric Adenocarcinoma. Front Oncol 10:609940 (2020). PubMed: 33469515
- Sun H et al. MicroRNA-124 regulates cell pyroptosis during cerebral ischemia-reperfusion injury by regulating STAT3. Exp Ther Med 20:227 (2020). PubMed: 33193841
- Gu C et al. Role of the thioredoxin interacting protein in diabetic nephropathy and the mechanism of regulating NOD-like receptor protein 3 inflammatory corpuscle. Int J Mol Med 43:2440-2450 (2019). PubMed: 31017263
- Lin C et al. Melatonin attenuates traumatic brain injury-induced inflammation: a possible role for mitophagy. J Pineal Res 61:177-86 (2016). PubMed: 27117839
- Lin L et al. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma. Sci Rep 6:26757 (2016). IHC . PubMed: 27225603
- Carbotti G et al. The IL-18 antagonist IL-18-binding protein is produced in the human ovarian cancer microenvironment. Clin Cancer Res 19:4611-20 (2013). IHC ; Human . PubMed: 23873689