Anti-IL-18 抗体 (ab191152)
Key features and details
- Rabbit polyclonal to IL-18
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-18 antibody
IL-18 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-18 -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human IL-18 aa 1 to the C-terminus. immunogen corresponding to mature protein (amino acids 37-193) of Human IL-18.
Database link: Q14116 -
ポジティブ・コントロール
- Human tonsil tissue, recombinant protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab191152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 22 kDa.
The detection limit for IL18 is approximately 1ng/lane under reducing conditions |
IHC-P | (2) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 22 kDa. The detection limit for IL18 is approximately 1ng/lane under reducing conditions |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. -
配列類似性
Belongs to the IL-1 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3606 Human
- Omim: 600953 Human
- SwissProt: Q14116 Human
- Unigene: 83077 Human
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別名
- Iboctadekin antibody
- IFN gamma inducing factor antibody
- IFN-gamma-inducing factor antibody
see all
画像
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Anti-IL-18 antibody (ab191152) at 0.5 µg/ml + Recombinant Human IL-18 Protein at 0.0005 µg
Predicted band size: 22 kDa -
IL-18 was detected in immunocytochemical section of blood cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab191152 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen
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Immunohistochemical analysis of formalin fixed paraffine embedded human tonsil tissue labeling IL-18 with ab191152 at 1 µg/ml
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab191152 は 17 報の論文で使用されています。
- Van Nieuwenhove E et al. Phenotypic analysis of Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis patients during treatment. Rheumatology (Oxford) N/A:N/A (2021). PubMed: 33693560
- Wang Y et al. Dihydroartemisinin inhibits activation of the AIM2 inflammasome pathway and NF-?B/HIF-1a/VEGF pathway by inducing autophagy in A431 human cutaneous squamous cell carcinoma cells. Int J Med Sci 18:2705-2715 (2021). PubMed: 34104103
- Suárez-Barrio C et al. Plasma Rich in Growth Factors Promotes Autophagy in ARPE19 Cells in Response to Oxidative Stress Induced by Blue Light. Biomolecules 11:N/A (2021). PubMed: 34203504
- Hofbauer D et al. β2-microglobulin triggers NLRP3 inflammasome activation in tumor-associated macrophages to promote multiple myeloma progression. Immunity 54:1772-1787.e9 (2021). PubMed: 34289378
- Zrzavy T et al. Neuropathological Variability within a Spectrum of NMDAR-Encephalitis. Ann Neurol 90:725-737 (2021). PubMed: 34562035