Anti-IL-17A 抗体 [EPR21776] (ab218013)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21776] to IL-17A
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-17A antibody [EPR21776]
IL-17A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21776] to IL-17A -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: EL4 cells treated with PMA, ionomycin (ab120116) and Brefeldin A, whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21776 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab218013の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 14,17 kDa (predicted molecular weight: 17 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 14,17 kDa (predicted molecular weight: 17 kDa). |
ターゲット情報
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機能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
組織特異性
Restricted to activated memory T-cells. -
配列類似性
Belongs to the IL-17 family. -
翻訳後修飾
Found both in glycosylated and nonglycosylated forms. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16171 Mouse
- SwissProt: Q62386 Mouse
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別名
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
画像
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All lanes : Anti-IL-17A antibody [EPR21776] (ab218013) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin (ab120116) for 6 hours, then with 500 ng/ml Brefeldin A for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 14,17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer and concentration: 5% NFDM/TBST
Expression of IL-17A can be induced by PMA and Ionomycin treatment (PMID 28382171).
The expression profile is consistent with the literature (PMID 9764847).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab218013 は 1 報の論文で使用されています。
- Peng S et al. Recombinant programmed cell death 1 inhibits psoriatic inflammation in imiquimod-treated mice. Int J Mol Med 46:869-879 (2020). PubMed: 32468061