Anti-IL-13 抗体 (ab9576)
Key features and details
- Rabbit polyclonal to IL-13
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human
- Isotype: unknown
製品の概要
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製品名
Anti-IL-13 antibody
IL-13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-13 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)
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ポジティブ･コントロール
- WB: Recombinant human IL-13 protein (ab9577).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40 -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.2 µg/ml. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution). |
ターゲット情報
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機能
Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages. -
関連疾患
Allergic rhinitis -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3596 Human
- Omim: 147683 Human
- SwissProt: P35225 Human
- Unigene: 845 Human
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別名
- Allergic rhinitis antibody
- ALRH antibody
- BHR 1 antibody
see all
画像
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Western blot - Anti-IL-13 antibody (ab9576)This image is courtesy of an Abreview submitted by Miss Fenny ChongAll lanes : Anti-IL-13 antibody (ab9576) at 1/3000 dilution
Lane 1 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.2 µg
Lane 2 : E. coli expressed human recombinant protein IL-13 from supplier 1 at 0.3 µg
Lane 3 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.2 µg
Lane 4 : E. coli expressed human recombinant protein IL-13 from supplier 2 at 0.3 µg
Lane 5 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.2 µg
Lane 6 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.3 µg
Lane 7 : E. coli expressed human recombinant protein IL-13 from supplier 3 at 0.4 µg
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 26 kDa (possible dimer)
Exposure time: 5 minutes15% denaturing gel. Blot was blocked with 2% milk for 16 hours at 4°C and incubated with the primary antibody for 1 hour at 25°C.
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Sandwich ELISA - Anti-IL-13 antibody (ab9576)Calibration curve of a sandwich assay for IL-13 using ab9576 as a detection antibody at a concentration of 0.5μg/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab9576 は 2 報の論文で使用されています。
- Kadin ME et al. IL-13 is produced by tumor cells in Breast Implant Associated Anaplastic Large Cell Lymphoma: implications for pathogenesis. Hum Pathol N/A:N/A (2018). PubMed: 29689246
- Oue S et al. Increased IL-13 expression is independently associated with neo-osteogenesis in patients with chronic rhinosinusitis. J Allergy Clin Immunol 140:1444-1448.e11 (2017). PubMed: 28601687
