Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 20 min, 100C
Permeabilization
No
Specification
intestine
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/800
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit anti-mouse IgG, Leica refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
many antigen retrieval regimes tested - citrate gave best signal/background.
Located in epithelial cells of crypts. Interestingly smooth muscle of circular and longitudinal layers also strongly stained
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 12 2011