Anti-IL-12 抗体 (ab9992)
Key features and details
- Goat polyclonal to IL-12
- Suitable for: ELISA, IHC-P, Sandwich ELISA, Neutralising, WB
- Reacts with: Human
- Isotype: unknown
製品の概要
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製品名
Anti-IL-12 antibody
IL-12 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IL-12 -
由来種
Goat -
アプリケーション
適用あり: ELISA, IHC-P, Sandwich ELISA, Neutralising, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human IL-12. p35: P29459 p40: P29460
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ポジティブ･コントロール
- WB: Recombinant human IL-12 p40 protein (ab9993).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
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| IHC-P |
Use at an assay dependent concentration.
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| Sandwich ELISA |
Use at an assay dependent concentration.
To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
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| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. |
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IHC-P
Use at an assay dependent concentration. |
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Sandwich ELISA
Use at an assay dependent concentration. To detect hIL-12 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83448 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-12. |
|
Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ターゲット情報
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機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161560 Human
- Omim: 161561 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
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別名
- CLMF antibody
- CLMF p35 antibody
- CLMF p40 antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (5)
ab9992 は 5 報の論文で使用されています。
- Leng H et al. Effects of aloe polysaccharide, a polysaccharide extracted from Aloe vera, on TNF-a-induced HaCaT cell proliferation and the underlying mechanism in psoriasis. Mol Med Rep 18:3537-3543 (2018). PubMed: 30066937
- Kang H et al. Puerarin inhibits M2 polarization and metastasis of tumor-associated macrophages from NSCLC xenograft model via inactivating MEK/ERK 1/2 pathway. Int J Oncol 50:545-554 (2017). PubMed: 28101568
- Cousin C et al. The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation. Eur J Immunol 45:1772-82 (2015). PubMed: 25778793
- Al-Sammak F et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . PubMed: 24069393
- Schlaepfer E & Speck RF Anti-HIV activity mediated by natural killer and CD8+ cells after toll-like receptor 7/8 triggering. PLoS ONE 3:e1999 (2008). Neutralising ; Human . PubMed: 18431484
