Anti-IL-12 抗体 (ab9992)
製品の概要
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製品名
Anti-IL-12 antibody
IL-12 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IL-12 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WB, ELISA, Neutralisingmore details -
種交差性
交差種: Human -
免疫原
Highly pure (>98%) recombinant hIL-12 (human Interleukin-12)
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ポジティブ･コントロール
- Recombinant human IL-12 p40 protein (ab9993) can be used as a positive control in WB.
製品の特性
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製品の状態
Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
PBS, pH 7.4, no preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
unknown -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| ELISA | Use at an assay dependent concentration. To detect hIL-12 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. |
ターゲット情報
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機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161560 Human
- Omim: 161561 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
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別名
- CLMF antibody
- CLMF p35 antibody
- CLMF p40 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-12 antibody (ab9992)IHC image of ab9992 staining in human astrocytoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9992, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
参考文献
This product has been referenced in:
- Cousin C et al. The immunosuppressive enzyme IL4I1 promotes FoxP3(+) regulatory T lymphocyte differentiation. Eur J Immunol 45:1772-82 (2015). Read more (PubMed: 25778793) »
- Al-Sammak F et al. Gastric Epithelial Expression of IL-12 Cytokine Family in Helicobacter pylori Infection in Human: Is it Head or Tail of the Coin? PLoS One 8:e75192 (2013). IHC-P ; Human . Read more (PubMed: 24069393) »
