Anti-IL-10RB/IL-10R2 抗体 [MM0360-8R24] (ab89884)
Key features and details
- Mouse monoclonal [MM0360-8R24] to IL-10RB/IL-10R2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24]
IL-10RB/IL-10R2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MM0360-8R24] to IL-10RB/IL-10R2 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human IL-10RB/IL-10R2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered. -
ポリ/モノ
モノクローナル -
クローン名
MM0360-8R24 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89884の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 36 kDa).
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Detects a band of approximately 51 kDa (predicted molecular weight: 36 kDa). |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IL29. -
関連疾患
Defects in IL10RB are the cause of inflammatory bowel disease type 25 (IBD25) [MIM:612567]. It is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. -
配列類似性
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3588 Human
- Omim: 123889 Human
- SwissProt: Q08334 Human
- Unigene: 654593 Human
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別名
- Cd210 antibody
- CDw210b antibody
- CRF2 4 antibody
see all
画像
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Anti-IL-10RB/IL-10R2 antibody [MM0360-8R24] (ab89884) + Human Placenta Tissue Lysate
Predicted band size: 36 kDa -
Overlay histogram showing Jurkat cells stained with ab89884 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89884, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab89884 は 1 報の論文で使用されています。
- Chiappinelli KB et al. Inhibiting DNA Methylation Causes an Interferon Response in Cancer via dsRNA Including Endogenous Retroviruses. Cell 162:974-86 (2015). PubMed: 26317466