Biotin Anti-IL-10 抗体 (ab271261)
Key features and details
- Biotin Rabbit polyclonal to IL-10
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-IL-10 antibody
IL-10 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to IL-10 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Mouse, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Mouse IL-10 aa 1 to the C-terminus.
Database link: P18893 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271261の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
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別名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
画像
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Western blot analysis of varying levels of Recombinant human IL-10 protein using ab271261 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant IL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Mouse mIL-10 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271261. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/ well of recombinant mIL-10 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab271261 は 2 報の論文で使用されています。
- Song J et al. E3 Ligase FBXW7 Facilitates Mycobacterium Immune Evasion by Modulating TNF-α Expression. Front Cell Infect Microbiol 12:851197 (2022). PubMed: 35651754
- Duan L et al. Extracellular vesicles derived from human placental mesenchymal stem cells alleviate experimental colitis in mice by inhibiting inflammation and oxidative stress. Int J Mol Med 46:1551-1561 (2020). PubMed: 32945344