Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin)
Permeabilization
Yes - 0.5% Triton TBS Ca/Azide
Specification
Human skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA TBS Ca/Azide
Dilution
1/200
Secondary antibody
Dilution
1/800
Name
Non-Abcam antibody was used: Invitrogen Goat anti-rabbit Alexa 568 (A11011)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
IL-10 (red) stain in normal human skin at 20x magnification, counterstained with DAPI. Antibody works well for IHC-Fr/IF, but required 4C O/N incubation to achieve desirable staining quality
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投稿 Jul 18 2018