Anti-IL-10 抗体 (ab34843)
製品の概要
-
製品名
Anti-IL-10 antibody
IL-10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-10 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Fr, ELISA, IHC-P, ICC/IF, Neutralising, RIAmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human IL-10.
-
ポジティブ･コントロール
- WB: 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS); IL-1 stimulated IL- 10 production from fibroblasts, recombinant protein
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
精製度
Whole antiserum -
特記事項（精製）
ab34843 antiserum has been sterile filtered and heated to 56°C for 30 minutes. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab34843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
|
| IHC-Fr | 1/400. | |
| ELISA | 1/20000 - 1/100000. (as detection antibody, using a monoclonal antibody for capture). |
|
| IHC-P | 1/400. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Neutralising | Use at an assay dependent concentration. | |
| RIA | Use at an assay dependent concentration. |
ターゲット情報
-
機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
-
別名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-10 antibody (ab34843)This image is courtesy of an anonymous Abreviewab34843 staining IL-10 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody (ab34843)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL-10 with ab34843.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/250) was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Castrogiovanni P et al. Moderate Physical Activity as a Prevention Method for Knee Osteoarthritis and the Role of Synoviocytes as Biological Key. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30691048) »
- Vartina E et al. Inflammatory cytokines and antimicrobial peptides in acquired heart diseases. Histol Histopathol N/A:18091 (2019). Read more (PubMed: 30734265) »
