製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to IL-10
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-Fr, ELISA, IHC-P, ICC/IF, Neutralising, RIAmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human IL-10.

  • ポジティブ・コントロール

    • WB: 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC)(PBMC stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS); IL-1 stimulated IL- 10 production from fibroblasts, recombinant protein

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.

In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.

IHC-Fr 1/400.
ELISA 1/20000 - 1/100000.

(as detection antibody, using a monoclonal antibody for capture).

IHC-P 1/400.
ICC/IF Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 組織特異性

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 配列類似性

    Belongs to the IL-10 family.
  • 細胞内局在

    Secreted.
  • Information by UniProt
  • 参照データベース

  • 別名

    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

画像

  • ab34843 staining IL-10 in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL-10 with ab34843.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25&degC. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4&degC. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

参考文献

This product has been referenced in:

  • Castrogiovanni P  et al. Moderate Physical Activity as a Prevention Method for Knee Osteoarthritis and the Role of Synoviocytes as Biological Key. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30691048) »
  • Vartina E  et al. Inflammatory cytokines and antimicrobial peptides in acquired heart diseases. Histol Histopathol N/A:18091 (2019). Read more (PubMed: 30734265) »
See all 43 Publications for this product

レビューと Q&A

1-10 of 26 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb
Blocking step
Innovex Background Buster as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Aug 20 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue (intestine)
Specification
intestine

Abcam user community

Verified customer

投稿 Sep 05 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Human skin)
Permeabilization
Yes - 0.5% Triton TBS Ca/Azide
Specification
Human skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jul 18 2018

Application
Western blot
Sample
Cynomolgus monkey Tissue lysate - whole (retina)
Gel Running Conditions
Reduced Denaturing (4-15% gel)
Loading amount
50 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

投稿 Nov 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

投稿 Nov 02 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human head & neck squamous cell carcinoma)
Permeabilization
Yes - Triton X-100 (0.1%) for 5 min
Specification
Human head & neck squamous cell carcinoma
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 29 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Permeabilization
Yes - 0.1% Triton X-100 in PBS, 3min
Specification
Human peripheral blood mononuclear cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jan 28 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Specification
Colon
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Sep 10 2012

Answer

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

ab34843, rabbit polyclonal anti-IL10,is the right choicesince it has been tested and is guaranteed to work in Human samples, in IHC-P and IHC-Fr. https://www.abcam.com/ab34843.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Answer

Thank you for contacting us.

I have attached the filled out Exel spreadsheet with all the requested information. To be fair, all the information asked could be answered with a look on the datasheet...

In addition, I had a look through our tissue slices for her IHC-P experiments. Instead of the Tonsils she can use Lymphknot (as we do not have Tonsil slides).

https://www.abcam.com/index.html?datasheet=4350 (or use the following: https://www.abcam.com/index.html?datasheet=4350). PC for ab25101and ab10563

https://www.abcam.com/index.html?datasheet=4348 (or use the following: https://www.abcam.com/index.html?datasheet=4348). PC for ab34843

https://www.abcam.com/index.html?datasheet=4372 (or use the following: https://www.abcam.com/index.html?datasheet=4372). PC for ab37158

Read More

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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