Anti-IL-10 抗体 (ab34843)

Reviews (13)Q&A (16)References (48)
Western blot - Anti-IL-10 antibody (ab34843)
  • Western blot - Anti-IL-10 antibody (ab34843)

Key features and details

  • Rabbit polyclonal to IL-10
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-IL-10 antibody [EP1115Y] (ab52909)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-IL-10 antibody
    IL-10 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to IL-10

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human IL-10.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab34843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.

In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule.

ターゲット情報

  • 機能

    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

  • 組織特異性

    Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

  • 配列類似性

    Belongs to the IL-10 family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    画像

    • Western blot - Anti-IL-10 antibody (ab34843)
      Western blot - Anti-IL-10 antibody (ab34843)
      Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein

      Secondary
      Goat anti-rabbit at 1/20000 dilution

      Predicted band size: 21 kDa



      4-20% reducing gel.
      Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.
      Dilution buffer: 3% BSA-TBS.
      Incubated O/N at 4°C.
    • Western blot - Anti-IL-10 antibody (ab34843)
      Western blot - Anti-IL-10 antibody (ab34843)
      Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng

      Secondary
      Peroxidase conjugated secondary at 1/40000 dilution

      Predicted band size: 21 kDa

    参考文献 (48)

    ab34843 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab34843 は 48 報の論文で使用されています。

    • Duan X & Qin G Notch inhibitor mitigates renal ischemia-reperfusion injury in diabetic rats. Mol Med Rep 21:583-588 (2020). PubMed: 31974606
    • Shigeoka M  et al. CD163+ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia. Clin Exp Dent Res 5:627-637 (2019). PubMed: 31890299
    • Chen S  et al. Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice. Acta Biomater 97:187-199 (2019). PubMed: 31386930
    • Li M  et al. Losmapimod Protected Epileptic Rats From Hippocampal Neuron Damage Through Inhibition of the MAPK Pathway. Front Pharmacol 10:625 (2019). PubMed: 31231220
    • Banerjee J  et al. Delivery of silver sulfadiazine and adipose derived stem cells using fibrin hydrogel improves infected burn wound regeneration. PLoS One 14:e0217965 (2019). PubMed: 31194776
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 29 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (spinal cord transversal section)
    Permeabilization
    Yes - 0,01% Triton-X 100
    Specification
    spinal cord transversal section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Tamas Bellak

    Verified customer

    投稿 Dec 01 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica BOND ER2
    Permeabilization
    No
    Specification
    tonsil
    Fixative
    Formaldehyde
    Read More

    Mr. Masahiro Hirata

    Verified customer

    投稿 Sep 09 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (CT26 tumor taken out Balb/C mice)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Ph6
    Permeabilization
    No
    Specification
    CT26 tumor taken out Balb/C mice
    Blocking step
    (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 15 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    MDA-MB-231 (ATCC® HTB-26™) tumor taken out of Balb
    Blocking step
    Innovex Background Buster as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue (intestine)
    Specification
    intestine
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 05 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-10 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Human skin)
    Permeabilization
    Yes - 0.5% Triton TBS Ca/Azide
    Specification
    Human skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 18 2018

    Western blot abreview for Anti-IL-10 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cynomolgus monkey Tissue lysate - whole (retina)
    Gel Running Conditions
    Reduced Denaturing (4-15% gel)
    Loading amount
    50 µg
    Specification
    retina
    Blocking step
    Milk as blocking agent for 1 hour(s) and 45 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 18 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-10 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human head & neck squamous cell carcinoma)
    Permeabilization
    Yes - Triton X-100 (0.1%) for 5 min
    Specification
    Human head & neck squamous cell carcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 29 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human peripheral blood mononuclear cells)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS, 3min
    Specification
    Human peripheral blood mononuclear cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 28 2013

    1-10 of 29 Abreviews or Q&A

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