Application
Western blot
Sample
Rat Cell lysate - whole cell (Macrophages and dentritic cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
15 µg
Specification
Macrophages and dentritic cells
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Concentration
0.1 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 31, 17 kDa Non-specific: non kDa
Additional data
Additional Notes
Works great even for the detection of endogenous rat IL-1b via western blotting. It can detect both the proIL-1b (p31) and IL-1b (p17).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 05 2012