Anti-IL-1 beta 抗体 (ab9787)

Reviews (3)Q&A (17)References (53)

Key features and details

  • Rabbit polyclonal to IL-1 beta
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Rat
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-IL-1 beta antibody
    IL-1 beta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to IL-1 beta

  • 由来種

    Rabbit

  • 特異性

    This antibody is used in our Rat IL-1beta EDK and was tested against the following rat growth factors at 50ng/ml to determine if there was any cross reactivity: GM-CSF, IL1alpha, IL-2, IL-4, IL-10, SCF, & TNFalpha. No significant cross reactivity was detected. The antibody has not been tested against endogenous IL1beta, only recombinant protein and we cannot guarantee it will detect endogenous protein.

  • アプリケーション

    適用あり: WB, Sandwich ELISAmore details

  • 種交差性

    交差種: Rat

  • 免疫原

    Recombinant full length protein corresponding to Rat IL-1 beta aa 2-268.
    Sequence:

    MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS


    Database link: Q63264

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Recombinant rat IL-1 beta (lower limit of detection: 1.95 ng/lane)

  • 特記事項

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab156791

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa.

To detect Rat IL-1β by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-1β is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect Rat IL-1 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9787 is required. This antigen affinity purified antibody, in conjunction with a ab245863 detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.

ターゲット情報

  • 機能

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.

  • 組織特異性

    Expressed in activated monocytes/macrophages (at protein level).

  • 配列類似性

    Belongs to the IL-1 family.

  • 翻訳後修飾

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

  • 細胞内局在

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Catabolin antibody
      • H1 antibody
      • IFN beta inducing factor antibody
      • IL 1 antibody
      • IL 1 beta antibody
      • IL-1 beta antibody
      • IL1 antibody
      • IL1 BETA antibody
      • IL1B antibody
      • IL1B_HUMAN antibody
      • IL1F2 antibody
      • Interleukin 1 beta antibody
      • Interleukin 1 beta precursor antibody
      • interleukin 1, beta antibody
      • Interleukin-1 beta antibody
      • OAF antibody
      • Osteoclast activating factor antibody
      • OTTHUMP00000162031 antibody
      • Preinterleukin 1 beta antibody
      • Preinterleukin beta antibody
      • Pro interleukin 1 beta antibody
      see all

    画像

    • Sandwich ELISA - Anti-IL-1 beta antibody (ab9787)
      Sandwich ELISA - Anti-IL-1 beta antibody (ab9787)

      This antigen affinity purified antibody, in conjunction with a suitable detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-1 beta.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (53)

    ab9787 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9787 は 53 報の論文で使用されています。

    • Ma H  et al. Osteoarthritis is Prevented in Rats by Verbascoside via Nuclear Factor kappa B (NF-?B) Pathway Downregulation. Med Sci Monit 26:e921276 (2020). PubMed: 32249762
    • Wang J  et al. Suberoylanilide hydroxamic acid alleviates orthotopic liver transplantation-induced hepatic ischemia-reperfusion injury by regulating the AKT/GSK3ß/NF-?B and AKT/mTOR pathways in rat Kupffer cells. Int J Mol Med 45:1875-1887 (2020). PubMed: 32236599
    • Luo J  et al. Chlorogenic acid attenuates cyclophosphamide-induced rat interstitial cystitis. Life Sci 254:117590 (2020). PubMed: 32220624
    • Choi SR  et al. Spinal Interleukin-1ß Inhibits Astrocyte Cytochrome P450c17 Expression Which Controls the Development of Mechanical Allodynia in a Mouse Model of Neuropathic Pain. Front Mol Neurosci 12:153 (2019). PubMed: 31281242
    • Wang Y  et al. Three Toxoplasma gondii Dense Granule Proteins Are Required for Induction of Lewis Rat Macrophage Pyroptosis. MBio 10:N/A (2019). PubMed: 30622189
    View all Publications for this product

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 20 Abreviews or Q&A

    Western blot abreview for Anti-IL-1 beta antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Purified protein (rat hippocampus tissue)
    Gel Running Conditions
    Non-reduced Denaturing (SDS-polyacrylamide)
    Loading amount
    50 µg
    Treatment
    RIPA buffer
    Specification
    rat hippocampus tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 12 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-IL-1 beta antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton-X100 in blocking buffer
    Specification
    Spinal cord
    Blocking step
    normal goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 04 2012

    Western blot abreview for Anti-IL-1 beta antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Macrophages and dentritic cells)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Specification
    Macrophages and dentritic cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 05 2012

    Question

    I would like to test ab9787 for detection of endogenous rat protein via western blotting.

    Read More
    Answer


    I am very pleased to hear you would like to accept our offer and test ab9787 in WB (endogenous protein). This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Thank you for youremail.
    But it seems to write requesting date notexpiration date.
    Please check again.

    Best regards

    Read More
    Answer

    Thank you for your message.

    My colleague Padamjeet is out of office today. I can confirm that the expiration date: 17-12-2012.

    Apologiesfor this mistake. If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Dear technical team,
    Our customer would like to purchase ab9787 toWB, IHC-Fr test and his sample is mouse.
    Let me know if you can give discount code for this sample.

    Read More
    Answer


    Expiration date: 17-08-2012

    I am very pleased to hear you would like to accept our offer and test ab9787 in [Mouse]. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for [Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Question

    Dear technical team,

    Our customer would like to know the detailed information of ab9722 and ab9787immunogen.
    The customer has rat and mouse samples so, if it is possible, let me know the homology between two species.
    Thank you in advance.

    Best regards,

    Read More
    Answer

    Thank you for your enquiry.

    As far as we are aware, ab9722 IL1 beta antibodyhas been tested and guaranteed in mouse but nottested in rat. the other ab9787IL1 beta antibody is tested and guaranteed for rat but not tested in mouse.

    As requested, I can confirm the following alignments:

    ab9722 Anti-IL1 beta antibody
    Already tested mouse.Alignment to rat: 89%

    ab9787 Anti-IL1 beta antibody
    Already tested rat.Alignment to mouse: 90%

    These alignments are high, so theoretically it is likely that ab9722 will detect in rat and that ab9787 will detect in mouse which I hope is good news for your customer. However, please note we are not able to guarantee this reactivity without further testing. If the customerwould like to test the antibody in this untested species, please contact me again by replying to this message prior to the purchase asthey may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

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    Question

    I have a follow-up question. Thank for the new lot of IL-1B - however, the new lot failed to fix our problem of having 1 band at 23 kDa. So we decided to run a loading control with pure IL-1beta we had in our lab. In the first 2 wells, I have 100 ng of IL-1B and 250 ng of IL-1B. In the third well I have 50 ng of IL-1B and 10 ug of a rat hippocampus sample that I have run before. In the fourth and fifth row I have 10 ug of rat hippocampus. We do see a band around 15-17 kDa, but there are several other bands in the wells with strictly IL-1B. Can you tell me what these other bands could be? I did my same protocol - with fresh beta mercaptoethanol and heated to 95 degrees for 5 minutes on a 4-20% gel and blocked in 5% filtered nonfat dry milk with the original antibody of IL-1B and with anti-rabbit secondary at 1:75,000. Please let me know if you need anymore information.

    Read More
    Answer

    Thank you for your reply.


    Unfortunately I am not sure what the identity of the extra bands might be. The protein you are using is not 100% pure so possibly other contaminants or strong multimers? It is reassuring that the antibody is recognizing the purified protein in your first few wells. It is very possible that the protein in the endogenous sample is simply running slightly higher due to a variety of factors, such as modifications, charge, etc.


    I do feel confident that the antibody is recognizing the correct protein. I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Question

    Thank you for looking into my inquiry. I would definitely like to try a different lot of the same antibody IL-1beta (ab9787) . If you need any other information, please let me know.

    Read More
    Answer

    Thank you for your reply.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXX.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Do you have any updates from the lab regarding the band detected by this antibody in my samples at 23kDa?

    Read More
    Answer

    Thank you for your patience as I have investigated this issue further with the laboratory.


    Based on your results and those obtained in our own laboratory it is not clear why this antibody is detecting a 23kDa band in your samples. I am happy to offer another lot or another antibody to this target as a replacement or instead a credit. Please let me know which you would prefer along with your previous order number.


    I look forward to your reply so that I may assist you further. Please do not hesitate to contact me if you have any additional questions.

    Read More

    1-10 of 20 Abreviews or Q&A

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