Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
lung
Blocking step
Hydrogen Peroxide as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
formalin 10%
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Phosphate Buffered Saline
Concentration
1 µg/ml
Secondary antibody
Name
Non-Abcam antibody was used: histofine simple stain max po multi
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Expression intracellular, secreted. Nonspecific binding noted in nuclei in samples without additional staining with hematoxylin.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Zhanna Ustenko
Verified customer
投稿 Aug 25 2020