Application
Western blot
Sample
Human Tissue lysate - whole (head and neck cancer tissue)
Gel Running Conditions
Reduced Denaturing (12% SDS page gel)
Loading amount
30 µg
Specification
head and neck cancer tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 2-8°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-linked Antibody (cell signali
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL™ Select Western Blotting Detection R
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 18 2019