Application
Flow Cytometry
Sample
Human Cell (human monocytes)
Permeabilization
Yes - 0,1% Saponin / PBS
Gating Strategy
no gate
Specification
human monocytes
Preparation
Cell harvesting/tissue preparation method: isolated by gradient centrifugation and followed by magnetic isolation
Sample buffer: 0,1% Saponin /1% FCS/ PBS
Sample buffer: 0,1% Saponin /1% FCS/ PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
45 minute(s) · Temperature: 4°C · Diluent: 0,1% Saponin /1% FCS/ PBS
Dilution
1/500
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: goat anti-rabbit Alexa488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
1 = unstimulated Monocytes;
2 = Monocytes stimulated with LPS.
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投稿 Oct 08 2013