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  1. Link

    il-1-beta-antibody-ab2105.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-1 beta 抗体 (ab2105)

  • Datasheet
  • SDS
Reviews (23)Q&A (17)References (146)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)

Key features and details

  • Rabbit polyclonal to IL-1 beta
  • Suitable for: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFr
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-IL-1 beta antibody [RM1009] (ab283818)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-IL-1 beta antibody
    IL-1 beta 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to IL-1 beta
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFrmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Non human primates
  • 免疫原

    Recombinant full length protein corresponding to Human IL-1 beta aa 100 to the C-terminus. Produced in E.coli. MW of recombinant IL-1 beta 17kDa, mature chain without propeptide.
    Database link: P01584

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • IHC-P: human medullary lymph node
  • 特記事項

    caution, F(c) domain of rabbit IgG may interact with cells non-specifically.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 特記事項(精製)

    IgG from whole rabbit serum purified by DEAE fractionation.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators
    • Metabolism
    • Types of disease
    • Obesity
    • Neuroscience
    • Processes

関連製品

  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human IL-1 beta protein (Active) (ab259387)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2105の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
RIA
Use at an assay dependent concentration.
ELISA (1)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-Fr (2)
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.

1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band).

IP
Use at an assay dependent concentration.

1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background).

Neutralising
Use at an assay dependent concentration.

Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta.

Flow Cyt (1)
Use at an assay dependent concentration.

F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (9)
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28726778
特記事項
RIA
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.

1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band).

IP
Use at an assay dependent concentration.

1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background).

Neutralising
Use at an assay dependent concentration.

Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta.

Flow Cyt
Use at an assay dependent concentration.

F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 28726778

ターゲット情報

  • 機能

    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 組織特異性

    Expressed in activated monocytes/macrophages (at protein level).
  • 配列類似性

    Belongs to the IL-1 family.
  • 翻訳後修飾

    Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 細胞内局在

    Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Target information above from: UniProt accession P01584 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3553 Human
    • Omim: 147720 Human
    • SwissProt: P01584 Human
    • Unigene: 126256 Human
    • 別名

      • Catabolin antibody
      • H1 antibody
      • IFN beta inducing factor antibody
      • IL 1 antibody
      • IL 1 beta antibody
      • IL-1 beta antibody
      • IL1 antibody
      • IL1 BETA antibody
      • IL1B antibody
      • IL1B_HUMAN antibody
      • IL1F2 antibody
      • Interleukin 1 beta antibody
      • Interleukin 1 beta precursor antibody
      • interleukin 1, beta antibody
      • Interleukin-1 beta antibody
      • OAF antibody
      • Osteoclast activating factor antibody
      • OTTHUMP00000162031 antibody
      • Preinterleukin 1 beta antibody
      • Preinterleukin beta antibody
      • Pro interleukin 1 beta antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemical analysis of Formalin-fixed paraffin-embedded human medullary lymph node sections labeling IL-1 beta with ab2105. Peroxidase goat anti-rabbit at 1/10,000 was used as the secondary antibody. 
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)This image is courtesy of an Abreview submitted by Miss Silke Vorwald
      ab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.

      See Abreview

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (146)

    ab2105 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2105 は 146 報の論文で使用されています。

    • Zhao K  et al. Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration. Cell Prolif 54:e12941 (2021). PubMed: 33111436
    • Wei X  et al. Ultrasound-targeted microbubble destruction-mediated Galectin-7-siRNA promotes the homing of bone marrow mesenchymal stem cells to alleviate acute myocardial infarction in rats. Int J Mol Med 47:677-687 (2021). PubMed: 33416139
    • Xu J  et al. Network Pharmacology and Experimental Evidence Identify the Mechanism of Astragaloside IV in Oxaliplatin Neurotoxicity. Drug Des Devel Ther 15:99-110 (2021). PubMed: 33469263
    • Razak S  et al. Molecular docking, pharmacokinetic studies, and in vivo pharmacological study of indole derivative 2-(5-methoxy-2-methyl-1H-indole-3-yl)-N'-[(E)-(3-nitrophenyl) methylidene] acetohydrazide as a promising chemoprotective agent against cisplatin induced organ damage. Sci Rep 11:6245 (2021). PubMed: 33737575
    • Liang H  et al. TGR5 activation attenuates neuroinflammation via Pellino3 inhibition of caspase-8/NLRP3 after middle cerebral artery occlusion in rats. J Neuroinflammation 18:40 (2021). PubMed: 33531049
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 40 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain, stomach, esophagus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    Yes - Tween 20 0.1%
    Specification
    brain, stomach, esophagus
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 26 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (chondrocytes)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    No
    Specification
    chondrocytes
    Blocking step
    Bloxall and Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Merissa Olmer

    Verified customer

    投稿 Jun 08 2020

    Western blot abreview for Anti-IL-1 beta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human monocyte, adipose drived stem cells)
    Gel Running Conditions
    Reduced Denaturing (10% SDS page gel)
    Loading amount
    30 µg
    Treatment
    1uM LPS for 24hr
    Specification
    Human monocyte, adipose drived stem cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Sep 18 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (cartilage)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: hyaluronidase
    Permeabilization
    No
    Specification
    cartilage
    Blocking step
    h2o2, then serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    zinc buffered formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Colon)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Target Retrieval Solution, ph6.0
    Permeabilization
    No
    Specification
    Colon
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Pan Pan

    Verified customer

    投稿 Aug 19 2016

    Western blot abreview for Anti-IL-1 beta antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cynomolgus monkey Tissue lysate - whole (Macaque Retina Lysate)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    200 µg
    Specification
    Macaque Retina Lysate
    Blocking step
    Milk as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 15 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Muller Cell Line MIO-M1)
    Permeabilization
    Yes - 0.1% TritonX-100
    Specification
    Muller Cell Line MIO-M1
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 01 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cynomolgus monkey Tissue sections (Macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    Macaque retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 10 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (lung)
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Specification
    lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 14 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-1 beta antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
    Permeabilization
    Yes - 0,1% Saponin/PBS
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 11 2013

    1-10 of 40 Abreviews or Q&A

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