Anti-IKZF3 Degron tag 抗体 [1C3] - BSA and Azide free (ab269478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free
IKZF3 Degron tag 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISAmore details
適用なし: ICC -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate. ELISA: Human IKZF3 Degron tag.
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特記事項
ab269478 is the carrier-free version of ab269464.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1C3 -
アイソタイプ
IgG1
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269478の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
画像
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All lanes : Anti-IKZF3 Degron tag antibody [1C3] (ab269464) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate
Lane 2 : HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Exposure time: 10 secondsThis data was produced using ab269464, the same antibody clone but in a different formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab269478 は論文での使用が確認できていません。