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Anti-IKZF3 Degron tag 抗体 [1C3] - BSA and Azide free (ab269478)

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Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
  • ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
  • Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free
    IKZF3 Degron tag 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ELISAmore details
    適用なし: ICC

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate. ELISA: Human IKZF3 Degron tag.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269478 is the carrier-free version of ab269464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    1C3

  • アイソタイプ

    IgG1

関連製品

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC.

    • 画像

    • Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
      Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
      All lanes : Anti-IKZF3 Degron tag antibody [1C3] (ab269464) at 1/1000 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate
      Lane 2 : HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

      Exposure time: 10 seconds


      This data was produced using ab269464, the same antibody clone but in a different formulation.

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
      ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

      This data was produced using ab269464, the same antibody clone but in a different formulation.

      ab269464 used at 0-4000 ng/ml.

      Antigen (Human IKZF3 Degron tag) used at 1000 ng/ml.

      Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

    • Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
      Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269478 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269478 は論文での使用が確認できていません。

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