Anti-IKKi/IKKe 抗体 (ab7891)
Key features and details
- Rabbit polyclonal to IKKi/IKKe
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IKKi/IKKe antibody
IKKi/IKKe 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IKKi/IKKe -
由来種
Rabbit -
特異性
Recognises IKK epsilon and does not cross react with IKK alpha, beta or gamma. -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human IKKi/IKKe aa 701-716.
Sequence:NRIIERLNRVPAPPDV
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified IgG was prepared by affinity chromatography. -
一次抗体 備考
Nuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. IkB is phosphorylated by IkB kinase(IKK) complex that contains IKKa, IKKb, and IKKg. A novel molecule in the IKK complex was recently identified and designated IKKe/IKK-i. IKKe is required for the activation of NF-kB by PMA and T cell receptors but not by TNFa and IL-1. IKKe/IKK-i message is expressed in a variety of tissues and is inducible by TNFa, IL-1, and LPS. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab7891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
ターゲット情報
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機能
Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death. -
組織特異性
Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated.
Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > PML body. Targeting to PML nuclear bodies upon DNA damage is TOPORS-dependent. - Information by UniProt
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参照データベース
- Entrez Gene: 9641 Human
- Entrez Gene: 56489 Mouse
- Omim: 605048 Human
- SwissProt: Q14164 Human
- SwissProt: Q9R0T8 Mouse
- Unigene: 321045 Human
- Unigene: 386783 Mouse
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別名
- I kappa B kinase epsilon antibody
- I-kappa-B kinase epsilon antibody
- IkBKE antibody
see all
画像
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Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : Untreated Raw264.7 cell lysates
Lane 2 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 3 hrs
Lane 3 : Raw264.7 cell lysates treated with LPS (0.3ug/ml) for 6 hrs
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa -
Western blot - Anti-IKKi/IKKe antibody (ab7891)All lanes : Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SK-N-SH cell lysate
Lane 5 : 3T3/NIH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 80 kDa -
Western blot - Anti-IKKi/IKKe antibody (ab7891)Anti-IKKi/IKKe antibody (ab7891) at 1 µg/ml + Jurkat whole cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe antibody (ab7891)ab7891 at 10µg/ml staining IKK epsilon in human pancreatic tissue by IHC.
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Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)ICC/IF image of ab7891 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7891, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-IKKi/IKKe antibody (ab7891)Immunocytochemistry/Immunofluorecense analysis of HeLa cells with ab7891 at 20µg/ml.
プロトコール
データシートおよび資料
参考文献 (11)
ab7891 は 11 報の論文で使用されています。
- Yuan Y et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). PubMed: 30794866
- Wang X et al. Expression and prognostic role of IKBKE and TBK1 in stage I non-small cell lung cancer. Cancer Manag Res 11:6593-6602 (2019). PubMed: 31406474
- Xia H et al. An evolutionary NS1 mutation enhances Zika virus evasion of host interferon induction. Nat Commun 9:414 (2018). PubMed: 29379028
- House CD et al. I??e cooperates with either MEK or non-canonical NF-kB driving growth of triple-negative breast cancer cells in different contexts. BMC Cancer 18:595 (2018). PubMed: 29801480
- Lork M et al. Importance of Validating Antibodies and Small Compound Inhibitors Using Genetic Knockout Studies-T Cell Receptor-Induced CYLD Phosphorylation by IKKe/TBK1 as a Case Study. Front Cell Dev Biol 6:40 (2018). PubMed: 29755980
