Anti-IKB alpha 抗体 (ab7217)

Reviews (13)Q&A (1)References (41)

Key features and details

  • Rabbit polyclonal to IKB alpha
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-IKB alpha antibody
    IKB alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to IKB alpha

  • 由来種

    Rabbit

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant full length protein (Mouse).

製品の特性

ターゲット情報

  • 機能

    Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

  • 関連疾患

    Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant

  • 配列類似性

    Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.

  • 翻訳後修飾

    Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

  • 細胞内局在

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

参考文献 (41)

ab7217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7217 は 41 報の論文で使用されています。

  • Xie S  et al. Andrographolide Protects Against Adverse Cardiac Remodeling After Myocardial Infarction through Enhancing Nrf2 Signaling Pathway. Int J Biol Sci 16:12-26 (2020). PubMed: 31892842
  • Shi CX  et al. Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia-reperfusion rats. Mol Med Rep 21:123-130 (2020). PubMed: 31746402
  • Ren F  et al. NRSN2 promotes breast cancer metastasis by activating PI3K/AKT/mTOR and NF-?B signaling pathways. Oncol Lett 19:813-823 (2020). PubMed: 31885716
  • Liu J  et al. Notoginsenoside R1 protects human renal proximal tubular epithelial cells from lipopolysaccharide-stimulated inflammatory damage by up-regulation of miR-26a. Chem Biol Interact 308:364-371 (2019). PubMed: 31158334
  • Li C  et al. Swainsonine inhibits proliferation and collagen synthesis of NIH-3T3 cells by declining miR-21. Artif Cells Nanomed Biotechnol 47:2256-2264 (2019). PubMed: 31184213
View all Publications for this product

レビューと Q&A

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1-10 of 14 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-IKB alpha antibody

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Read More

Dr. Alain Chedotal

Verified customer

投稿 Feb 13 2015

Western blot abreview for Anti-IKB alpha antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Loading amount
20 µg
Specification
Liver
Gel Running Conditions
Non-reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More

Dr. Fabio Marongiu

Verified customer

投稿 Oct 11 2012

Question

merci de bien vouloir trouver ci-après la liste des anticorps pour commande, je vous serais reconnaissante de bien vouloir m’indiquer pour chaque ref citée le code de remise correspondant afin que je puisse passer la commande ferme.

Par ailleurs, les anticorps référencés ci-dessous, qui ont été acheté à un prix normal, n’ont pas répondu avec le robot SIMON, pourriez-vous envisager un geste commercial pour ces produits qui finalement ont été achetés en vain!

Read More
Answer

Codes offre de test :
ab32515 : *****
ab32096 : *****
ab7217 : *****
ab12135 : *****
ab1791 : *****

Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participer ànotre offre et tester les anticorps listés ci-dessusavec le robot Simon de Protein Simple.Ces codes de réductionsont utilisables sous 120 jours et pour l’achat d’autres anticorps primaires.

Vous devez dans un premier temps soumettre une Abreview avec vos résultats enspécifiant le code promotionnel correspondant à l'anticorps testé. Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de l'anticorps testé.

Concernantles produits pour lesquels vousavez déjà obtenu des résultats, merci dem'envoyer vos données, en échange nous vous attribueronsdes"Abpoints" utilisables pour des remisessur vos commandes ouen bons d'achatAmazon.

Je reste à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview je vous souhaite unebonne continuation dans vos recherches.

Read More

Western blot abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Pancreas)
Loading amount
200000 cells
Specification
Pancreas
Treatment
With and without TNF-a
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Read More

Abcam user community

Verified customer

投稿 Dec 01 2011

Immunohistochemistry - Free Floating abreview for Anti-IKB alpha antibody

 Good
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain sections)
Specification
Mouse brain sections
Read More

Abcam user community

Verified customer

投稿 Jan 12 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification
NIH3T3 Mouse Fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Jan 12 2011

Immunoprecipitation abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input
200 µg
Specification
NIH3T3 Mouse Fibroblasts
Immuno-precipitation step
Protein A/G
Read More

Abcam user community

Verified customer

投稿 Jan 11 2011

Western blot abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - other (NIH 3T3 Mouse Fibroblasts)
Loading amount
20 µg
Specification
NIH 3T3 Mouse Fibroblasts
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Dec 24 2010

Immunocytochemistry abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Human gastric carcinoma cells)
Specification
AGS Human gastric carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More

Abcam user community

Verified customer

投稿 Oct 26 2010

Immunoprecipitation abreview for Anti-IKB alpha antibody

 Good
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
200 µg
Specification
HeLa cells
Immuno-precipitation step
Protein A/G
Read More

Abcam user community

Verified customer

投稿 Oct 19 2010

1-10 of 14 Abreviews or Q&A

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