リコンビナントRabMAb

Anti-IGJ 抗体 [EPR23130-113] - BSA and Azide free (ab269860)

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Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
  • Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
  • Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23130-113] to IGJ - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-IGJ antibody [EPR23130-113] - BSA and Azide free
    IGJ 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23130-113] to IGJ - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human tonsil tissue lysate; Daudi, Raji and Ramos cell lysate. IHC-P: Human bladder cancer, spleen and colon tissue. ICC/IF: Daudi cells. IP: Human tonsil tissue lysate.

  • 特記事項

    ab269860 is the carrier-free version of ab269855. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269860 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23130-113

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269860 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also help to bind these immunoglobulins to secretory component.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • IGCJ antibody
      • IGJ_HUMAN antibody
      • Immunoglobulin J chain antibody
      • Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody
      • immunoglobulin joining chain antibody
      • JCH antibody
      • JCHAIN antibody
      • Joining chain of multimeric IgA and IgM antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Immunocytochemistry/ Immunofluorescence - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Daudi cells labeling IGJ with ab224687 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Daudi cells is observed. ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

      Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human bladder cancer is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human colon (PMID:12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling IGJ with ab269855 at 1/8000 dilution (0.06 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in plasma cells of human spleen (PMID:12389099) is observed. The section was incubated with ab269855 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

    • Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Immunoprecipitation - Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

      IGJ was immunoprecipitated from 0.35 mg human tonsil lysate with ab269855 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 269855 1/1000 dilution (0.48 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Human tonsil lysate 10 μg
      Lane 2: ab269855 IP in human tonsil lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269855 in human tonsil lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269855).

    • Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)
      Anti-IGJ antibody [EPR23130-113] - BSA and Azide free (ab269860)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269860 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269860 は論文での使用が確認できていません。

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