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Anti-IGHD 抗体 [IA6-2] - BSA and Azide free (ab269972)

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Western blot - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
  • Flow Cytometry - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
  • ELISA - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
  • Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IA6-2] to IGHD - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ELISA
  • Reacts with: Human, Recombinant fragment

製品の概要

  • 製品名

    Anti-IGHD antibody [IA6-2] - BSA and Azide free
    IGHD 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IA6-2] to IGHD - BSA and Azide free

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    The details of the immunogen for this antibody are not available.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: Human plasma and serum lysate. Flow Cyt: Human peripheral blood mononuclear cell (PBMC).

  • 特記事項

    ab269972 is the carrier-free version of ab235125. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    IA6-2

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ELISA
Recombinant fragment
Flow Cyt
Human
WB
Human
アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ELISA
Use at an assay dependent concentration.
特記事項
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
ELISA
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-Fr or IHC-P.

    • ターゲット情報

    • 関連性

      There are two named isoforms of IGHD produced by alternative splicing, a secreted form and a single pass type I membrane protein.

    • 細胞内局在

      Isoform 1: Secreted Isoform 2: Cell membrane; Single pass type I membrane protein.

    • 参照データベース

      • 別名

        • IGHD antibody
        • Constant region of heavy chain of IgD antibody
        • Ig delta chain C region antibody
        • Ig delta chain C region secreted form antibody
        • IgD antibody
        • Igh-5 antibody
        • Immunoglobulin delta antibody
        • Immunoglobulin heavy constant delta antibody
        • Immunoglobulin heavy delta chain antibody
        see all

      画像

      • Western blot - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
        Western blot - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
        All lanes : Anti-IGHD antibody [IA6-2] (ab235125) at 1.032 µg/ml

        Lane 1 : Human plasma lyaste
        Lane 2 : Human serum lyaste

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

        Predicted band size: 42 kDa


        Exposure time: 8 seconds


        This antibody recognize the heavy chain (~60 kDa) of IgD.

        Blocking/Dilution buffer: 5% NFDM/TBST.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

      • Flow Cytometry - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
        Flow Cytometry - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

        Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling IGHD with ab235125 at 1/500 dilution (2.064µg/ml) (Right panel) compared with a mouse monoclonal IgG isotype control (Left panel). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG (Left) or ab235125 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647. Gated on viable cells.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

      • ELISA - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
        ELISA - Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

        ELISA - Anti-IGHD antibody [IA6-2] (ab235125).

        ab235125 used at 0-4000 ng/ml.

        Antigen (Human IGD) used at 1000 ng/ml.

        Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235125).

      • Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)
        Anti-IGHD antibody [IA6-2] - BSA and Azide free (ab269972)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269972 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269972 は論文での使用が確認できていません。

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