リコンビナントRabMAb

Anti-IgG4 抗体 [EP4420] - BSA and Azide free (ab271883)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)
  • Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4420] to IgG4 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-IgG4 antibody [EP4420] - BSA and Azide free
    IgG4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP4420] to IgG4 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human spleen and Human plasma lysates; Human tonsil tissue.

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    ab271883 is the carrier-free version of ab109493. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP4420

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab271883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
IHC-P Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC or IP.

    • ターゲット情報

    • 関連性

      IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.

    • 細胞内局在

      Secreted

    • 参照データベース

      • 別名

        • Ig gamma 4 chain C region antibody
        • IGHG4 antibody
        • immunoglobulin heavy chain constant region gamma 4 antibody
        • Immunoglobulin heavy constant gamma 4 (G4m marker) antibody
        • MGC117419 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)

        ab109493 at 1/1000 dilution staining IgG4 in Paraffin embedded Human tonsil tissue.

        Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109493).
      • Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)
        Anti-IgG4 antibody [EP4420] - BSA and Azide free (ab271883)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab271883 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab271883 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab271883.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com