Anti-IGFBP3 抗体 (ab272857)
Key features and details
- Rabbit polyclonal to IGFBP3
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IGFBP3 antibody
IGFBP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IGFBP3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment within Human IGFBP3. The exact sequence is proprietary. Center region.
Database link: P17936 -
ポジティブ・コントロール
- WB: HEK-293T whole cell lysate. Mouse plasma. IHC-Fr: E13.5 rat brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272857の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000.
|
|
IHC-Fr |
1/100 - 1/1000.
|
特記事項 |
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WB
1/500 - 1/3000. |
IHC-Fr
1/100 - 1/1000. |
ターゲット情報
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
組織特異性
Expressed by most tissues. Present in plasma. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
発生段階
IGFBP3 levels are higher during extrauterine life and peak during puberty. -
ドメイン
The thyroglobulin type-1 domain mediates interaction with HN. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3486 Human
- Entrez Gene: 16009 Mouse
- Entrez Gene: 24484 Rat
- Omim: 146732 Human
- SwissProt: P17936 Human
- SwissProt: P47878 Mouse
- SwissProt: P15473 Rat
- Unigene: 450230 Human
see all -
別名
- Acid stable subunit of the 140 K IGF complex antibody
- Binding protein 29 antibody
- Binding protein 53 antibody
see all
画像
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Frozen sections of E13.5 rat brain tissue stained for IGFBP3 (green) using ab272857 at 1/250 dilution in immunohistochemical analysis.
Counterstain: beta Tubulin 3/ TUJ1, a mature neuron marker (red). Fluoroshield™ with DAPI (blue).
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Anti-IGFBP3 antibody (ab272857) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
HRP-conjugated anti-rabbit IgG antibody12% SDS PAGE
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Anti-IGFBP3 antibody (ab272857) at 1/1000 dilution + Mouse plasma at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272857 は論文での使用が確認できていません。