Anti-IGFBP1 抗体 [EPR14472(B)] (ab181141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14472(B)] to IGFBP1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-IGFBP1 antibody [EPR14472(B)]
IGFBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14472(B)] to IGFBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Human placenta lysate; HepG2 whole cell lysate (ab7900).
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14472(B) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
|
特記事項 |
---|
WB
1/1000 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
-
機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 3484 Human
- Entrez Gene: 16006 Mouse
- Entrez Gene: 25685 Rat
- Omim: 146730 Human
- SwissProt: P08833 Human
- SwissProt: P47876 Mouse
- SwissProt: P21743 Rat
- Unigene: 642938 Human
see all -
別名
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
画像
-
Anti-IGFBP1 antibody [EPR14472(B)] (ab181141) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Anti-IGFBP1 antibody [EPR14472(B)] (ab181141) at 1/5000 dilution + Human placenta lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (8)
ab181141 は 8 報の論文で使用されています。
- Wang J et al. Norepinephrine exposure restrains endometrial decidualization during early pregnancy. J Endocrinol 248:277-288 (2021). PubMed: 33434145
- Tang X et al. Insulin-like growth factor binding protein-1 regulates HIF-1α degradation to inhibit apoptosis in hypoxic cardiomyocytes. Cell Death Discov 7:242 (2021). PubMed: 34531382
- Huang F et al. Carbon disulfide induced decidualization disorder in the mice uterus at the window of implantation. Ecotoxicol Environ Saf 190:110069 (2020). PubMed: 31841894
- Li X et al. IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1 and IGFBP1. FEBS Open Bio 10:2687-2701 (2020). PubMed: 33064893
- Tan B et al. Matrix Metalloproteinase-11 Promotes Early Mouse Mammary Gland Tumor Growth through Metabolic Reprogramming and Increased IGF1/AKT/FoxO1 Signaling Pathway, Enhanced ER Stress and Alteration in Mitochondrial UPR. Cancers (Basel) 12:N/A (2020). PubMed: 32825455
- Wu X et al. Role of IGFBP1 in the senescence of vascular endothelial cells and severity of aging-related coronary atherosclerosis. Int J Mol Med 44:1921-1931 (2019). PubMed: 31545483
- Charni-Natan M et al. Various stress stimuli rewire the profile of liver secretome in a p53-dependent manner. Cell Death Dis 9:647 (2018). PubMed: 29844359
- Nielsen KO et al. Hepatitis B virus suppresses the secretion of insulin-like growth factor binding protein 1 to facilitate anti-apoptotic IGF-1 effects in HepG2 cells. Exp Cell Res 370:399-408 (2018). PubMed: 29981339