Anti-IGF2 抗体 (ab9574)
製品の概要
-
製品名Anti-IGF2 antibody
IGF2 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to IGF2
-
由来種Rabbit
-
アプリケーション適用あり: WB, ELISA, Neutralising, ICC/IF, IHC-Pmore details
-
種交差性交差種: Rat, Cow, Human
-
免疫原
Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
-
ポジティブ･コントロール
- ab9575 (Active IGF2 full length protein) can be used as a positive control.
-
特記事項
Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
製品の特性
-
製品の状態Lyophilised:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
-
保存方法Shipped at 4°C. Store at -20ºC.
-
バッファーPBS, pH 7.4, no preservative, sterile filtered
-
Concentration information loading... -
精製度Immunogen affinity purified
-
一次抗体 備考Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
軽鎖の種類unknown
-
研究分野
関連製品
-
Compatible Secondaries
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab9574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| ELISA | Use at an assay dependent concentration. To detect hIGF-2 by ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-2. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-2 (10.0 ng/ml), a concentration of 3.1 - 4.6 µg/ml of this antibody is required. | |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
-
機能The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. -
関連疾患Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
-
配列類似性Belongs to the insulin family.
-
翻訳後修飾O-glycosylated with a core 1 or possibly core 8 glycan.
-
細胞内局在Secreted.
- Information by UniProt
-
参照データベース
- Entrez Gene: 281240 Cow
- Entrez Gene: 3481 Human
- Entrez Gene: 24483 Rat
- Omim: 147470 Human
- SwissProt: P07456 Cow
- SwissProt: P01344 Human
- SwissProt: P01346 Rat
- Unigene: 272259 Human
see all -
別名
- C11orf43 antibody
- IGF 2 antibody
- IGF II antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2 antibody (ab9574)Immunohistochemical analysis of parafin embedded rat kidney tissue labelling IGF2 with ab9574 at 1ug/ml.
-
Western blot - Anti-IGF2 antibody (ab9574)Image courtesy of an anonymous Abreview.Anti-IGF2 antibody (ab9574) at 1/2000 dilution + whole tissue lysate prepared from murine liver at 40 µg
Secondary
HRP conjugated goat anti-rabbit polyclonal at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
-
Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody (ab9574)ICC/IF image of ab9574 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9574, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-IGF2 antibody (ab9574)Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
Lane 1: Marker
Lane 2: 250 ng
Lane 3: 125 ng
Lane 4: 62.5 ng
Lane 5: 31.25 ng
Lane 6: 15.625 ng
Lane 7: 7.8 ng
Lane 8: 3.9 ng -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2 antibody (ab9574)This image is courtesy of an anonymous Abreviewab9574 staining human tongue carcinoma tissue sections by IHC-P. For antigen retrieval sections were placed in a bath of 0.01M citric acid, pH6, and were heated on full power in a microwave for 5 minutes and then for a further 4 minutes. The slides were then cooled and placed in PBS, fixed with paraformaldehyde and blocked with BSA. The slides were incubated with the antibody for 18 hours. An HRP conjugated pig polyclonal antibody was used as the secondary. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2 antibody (ab9574)IHC-P of normal human placenta stained with ab9574. The recommended concentration ranges from 0.5 µg/ml-5.0 µg/ml. An HRP-labeled polymer detection system was used with DAB chromogen. Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K. Optimal concentrations and conditions may vary.
プロトコール
参考文献
This product has been referenced in:
- Van Arsdale AR et al. Insulin-like growth factor 2: a poor prognostic biomarker linked to racial disparity in women with uterine carcinosarcoma. Cancer Med 7:616-625 (2018). IHC-P ; Human . Read more (PubMed: 29455465) »
- Ireland L et al. Blockade of insulin-like growth factors increases efficacy of paclitaxel in metastatic breast cancer. Oncogene 37:2022-2036 (2018). IHC-P . Read more (PubMed: 29367764) »
