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  1. Link

    igf2-antibody-ab9574.pdf

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Signal Transduction Growth Factors/Hormones Insulin / Insulin-like
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Anti-IGF2 抗体 (ab9574)

  • Datasheet
  • SDS
Reviews (20)Q&A (21)References (92)

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Abpromise

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Sandwich ELISA - Anti-IGF2 antibody (ab9574)
  • Western blot - Anti-IGF2 antibody (ab9574)

Key features and details

  • Rabbit polyclonal to IGF2
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-IGF2 antibody [EPR12221] (ab177467)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-IGF2 antibody
    IGF2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to IGF2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, Sandwich ELISAmore details
  • 種交差性

    交差種: Recombinant fragment
  • 免疫原

    Recombinant full length protein corresponding to Human IGF2. Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).
    Database link: P01344

  • 特記事項

    Although some customers have been successful in IHC we no longer batch test in this application.


    Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Lyophilized:Reconstitute with 200µl of sterile water. The reconstituted antibody is stable for at least 2 weeks at 2-8C and at least 6 months at -20C
  • 保存方法

    Shipped at 4°C. Store at -20ºC.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 軽鎖の種類

    unknown
  • 研究分野

    • Signal Transduction
    • Growth Factors/Hormones
    • Insulin / Insulin-like
    • Cancer
    • Growth factors
    • Insulin and insulin-like
    • Developmental Biology
    • Post embryonic development
    • Growth hormones

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant human IGF2 protein (ab9575)
  • Recombinant Protein

    • Recombinant human IGF2 protein (ab9575)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9574の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (6)
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA
Use at an assay dependent concentration.

To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.

特記事項
WB
Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich ELISA
Use at an assay dependent concentration.

To detect hIGF-II by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83142, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-II.

ターゲット情報

  • 機能

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 関連疾患

    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • 配列類似性

    Belongs to the insulin family.
  • 翻訳後修飾

    O-glycosylated with a core 1 or possibly core 8 glycan.
  • 細胞内局在

    Secreted.
  • Target information above from: UniProt accession P01344 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 別名

    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

画像

  • Sandwich ELISA - Anti-IGF2 antibody (ab9574)
    Sandwich ELISA - Anti-IGF2 antibody (ab9574)

    ab9574 detecting IGF2 using sandwich ELISA

  • Western blot - Anti-IGF2 antibody (ab9574)
    Western blot - Anti-IGF2 antibody (ab9574)

    Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
    Lane 1: Marker
    Lane 2: 250 ng
    Lane 3: 125 ng
    Lane 4: 62.5 ng
    Lane 5: 31.25 ng
    Lane 6: 15.625 ng
    Lane 7: 7.8 ng
    Lane 8: 3.9 ng

     

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (92)

ab9574 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9574 は 92 報の論文で使用されています。

  • Li D  et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
  • Gao T  et al. Long non-coding RNA 91H regulates IGF2 expression by interacting with IGF2BP2 and promotes tumorigenesis in colorectal cancer. Artif Cells Nanomed Biotechnol 48:664-671 (2020). PubMed: 32070145
  • García-Huerta P  et al. Insulin-like growth factor 2 (IGF2) protects against Huntington's disease through the extracellular disposal of protein aggregates. Acta Neuropathol 140:737-764 (2020). PubMed: 32642868
  • Suzukawa AA  et al. Downregulation of IGF2 expression in third trimester placental tissues from Zika virus infected women in Brazil. J Infect 81:766-775 (2020). PubMed: 32987099
  • Zaanan A  et al. High IGF1R protein expression correlates with disease-free survival of patients with stage III colon cancer. Cell Oncol (Dordr) 43:237-247 (2020). PubMed: 31823290
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 41 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2 antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid based buffer
Permeabilization
Yes - 0.3% triton X-100 in PBS
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative
Paraformaldehyde
Read More

Huaxia Dong

Verified customer

投稿 Jun 15 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
Permeabilization
No
Specification
lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 21°C
Fixative
10% normal buffered formalin
Read More

Abcam user community

Verified customer

投稿 Nov 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

投稿 Sep 21 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% formalin
Read More

Abcam user community

Verified customer

投稿 Sep 21 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IGF2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate pH6
Permeabilization
No
Specification
tumor
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
10% normal buffered formalin
Read More

Abcam user community

Verified customer

投稿 Sep 21 2018

Western blot abreview for Anti-IGF2 antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
5 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 05 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Permeabilization
Yes - 0.1% triton X100
Specification
HEK293
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Jul 18 2017

Immunocytochemistry abreview for Anti-IGF2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (PULP)
Permeabilization
Yes - tritonx100
Specification
PULP
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde
Read More

Kyungjoo Seong

Verified customer

投稿 Nov 30 2016

Western blot abreview for Anti-IGF2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jun 02 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-IGF2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (primary hepatocytes)
Specification
primary hepatocytes
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Oct 01 2013

1-10 of 41 Abreviews or Q&A

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